• Publications
  • Influence
Simple and cost-effective isolation of monocytes from buffy coats.
We have optimized the procedure of monocyte isolation on a Percoll density gradient. The new procedure consists of three steps: (1) the isolation of MNC on a Ficoll density gradient; (2) theExpand
  • 144
  • 7
Bone tissue engineering with human stem cells
Treatment of extensive bone defects requires autologous bone grafting or implantation of bone substitute materials. An attractive alternative has been to engineer fully viable, biological bone graftsExpand
  • 165
  • 6
  • PDF
Covalent immobilization of antimicrobial agents on titanium prevents Staphylococcus aureus and Candida albicans colonization and biofilm formation.
OBJECTIVES Biofilm-associated implant infections represent a serious public health problem. Covalent immobilization of antimicrobial agents on titanium (Ti), thereby inhibiting biofilm formation ofExpand
  • 35
  • 1
Transfection of mammalian cells by the methods of receptor mediated gene transfer and particle bombardment.
The efficacy of currently developed methods for gene transfer into mammalian cells depends primarily on the transfection technique, and also on the type of targeted cells. Considering the importanceExpand
  • 9
  • 1
Protein synthesis of human articular chondrocytes cultured in vitro for autologous transplantation
Abstract Chondrocytes in hyaline cartilage produce typical matrix proteins, the most abundant of them being collagen type II and aggrecan. Chondrocytes in monolayer cell culture dedifferentiate andExpand
  • 10
  • 1
Testing the potency of anti‐TNF‐α and anti‐IL‐1β drugs using spheroid cultures of human osteoarthritic chondrocytes and donor‐matched chondrogenically differentiated mesenchymal stem cells
Inflammation plays a major role in progression of rheumatoid arthritis, a disease treated with antagonists of tumor necrosis factor‐alpha (TNF‐α) and interleukin 1β (IL‐1β). New in vitro testingExpand
  • 6
  • 1
Cryopreservation of Human Adipose-Derived Stem Cells in Combination with Trehalose and Reversible Electroporation
New cryopreservation approaches for medically applicable cells are of great importance in clinical medicine. Current protocols employ the use of dimethyl sulfoxide (DMSO), which is toxic to cells andExpand
  • 18
  • PDF
Evaluation of a fibrin-based skin substitute prepared in a defined keratinocyte medium.
The purpose of this study was to evaluate the influence of fibrin glue and aprotinin on the growth of adult human skin keratinocytes in defined serum-free conditions. The keratinocytes were culturedExpand
  • 22
Determination of optimal transport conditions for biopsies of human articular cartilage as well as for suspensions of cultured chondrocytes used in autologous transplantation
Abstract Autologous transplantation of chondrocytes is currently being promoted as a novel approach for the treatment of deep cartilage lesions. Briefly, the method involves enzyme-mediated releaseExpand
  • 3
Vitamin C induced apoptosis in human articular chondrocytes
Abstract Chondrocytes present in articular cartilage survive as a resident cell population throughout the lifespan of the individual organism. However, articular chondrocytes as other cells alsoExpand
  • 4