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Establishment of medaka (Oryzias latipes) transgenic lines with the expression of green fluorescent protein fluorescence exclusively in germ cells: A useful model to monitor germ cells in a live
We have generated transgenic medaka (teleost, Oryzias latipes), which allow us to monitor germ cells by green fluorescent protein (GFP) fluorescence in live specimens. Two medaka strains, himedakaExpand
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The see-through medaka: A fish model that is transparent throughout life
The see-through medaka is a vertebrate model with a transparent body in the adult stage, as well as during the embryonic stages, that was generated from a small laboratory fish, medaka (OryziasExpand
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Targeted mutagenesis using CRISPR/Cas system in medaka
ABSTRACT Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system-based RNA-guided endonuclease (RGEN) has recently emerged as a simple and efficient tool forExpand
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Degradation of Oval‐Filefish Meat Gel Caused by Myofibrillar Proteinase(s)
There exists some discrepancy about the distribution of the gel degradation property in muscle cells among fish species. Two possible cause are assumed; methods to separate the sarcoplasmic andExpand
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Efficient Targeted Mutagenesis in Medaka Using Custom-Designed Transcription Activator-Like Effector Nucleases
Transcription activator-like effector nucleases (TALENs) have become powerful tools for targeted genome editing. Here we demonstrate efficient targeted mutagenesis in medaka (Oryzias latipes), whichExpand
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In vivo visual reporter system for detection of estrogen-like substances by transgenic medaka.
Detection of endocrine disrupting chemicals, in particular, environmental estrogens with living organisms, has many advantages if compared to chemical analysis. The screening of novel pollutants withExpand
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Targeted disruption of exogenous EGFP gene in medaka using zinc‐finger nucleases
Zinc‐finger nucleases (ZFNs) are artificial enzymes that create site‐specific double‐strand breaks and thereby induce targeted genome editing. Here, we demonstrated successful gene disruption inExpand
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Leucophores are similar to xanthophores in their specification and differentiation processes in medaka
Significance Body color plays an important role in the diversity and speciation of vertebrates. In this paper, we revealed that three loci in medaka, leucophore free (lf), lf-2, and white leucophore,Expand
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DMY gene induces male development in genetically female (XX) medaka fish
Although the sex-determining gene SRY/Sry has been identified in mammals, homologues and genes that have a similar function have yet to be identified in nonmammalian vertebrates. Recently, DMY (theExpand
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