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Angucyclines: Biosynthesis, mode-of-action, new natural products, and synthesis.
The main focus of this article is on new synthetic approaches and biosynthetic investigations, most of which were published between 1997 and 2010, but go beyond, e.g. for some biosyntheses all the way back to the 1980s, to provide the necessary context of information. Expand
A comprehensive review of glycosylated bacterial natural products.
This comprehensive analysis of 15 940 bacterial natural products revealed 3426 glycosides containing 344 distinct appended carbohydrates and highlights a range of unique opportunities for future biosynthetic study and glycodiversification efforts. Expand
Isolation and characterization of the tobramycin biosynthetic gene cluster from Streptomyces tenebrarius.
The biosynthetic gene cluster for tobramycin, a 2-deoxystreptamine-containing aminoglycoside antibiotic, was isolated from Streptomyces tenebrarius ATCC 17920 and it was demonstrated that tbmA encodes DOI synthase by in vitro enzyme assay of the purified protein. Expand
Multi-oxygenase complexes of the gilvocarcin and jadomycin biosyntheses.
It is suggested that jadomycin and gilvocarcin biosynthesis diverge later in the respective pathways than previously assumed, and that the 5,6-bond cleavage necessitates the formation of multi-oxygenase complexes consisting of at least 3 and 4 enzymes, respectively. Expand
Molecular cloning and characterization of a 2-deoxystreptamine biosynthetic gene cluster in gentamicin-producing Micromonospora echinospora ATCC15835.
The organization of the 2-deoxystreptamine (DOS) biosynthetic gene cluster of Micromonospora echinospora has been determined and both gtmA and gtmJ are implicated in the biosynthesis of gentamicin and in resistance to it, respectively. Expand
Baeyer-Villiger C-C bond cleavage reaction in gilvocarcin and jadomycin biosynthesis.
GilOII has been unambiguously identified as the key enzyme performing the crucial C-C bond cleavage reaction responsible for the unique rearrangement of a benz[a]anthracene skeleton to theExpand
Cloning and Characterization of the Ravidomycin and Chrysomycin Biosynthetic Gene Clusters
The ravidomycin glycosyltransferase (RavGT) appears to be able to transfer both amino‐ and neutral sugars, exemplified through the structurally distinct 6‐membered D‐ravidosamine and 5‐members D‐fucofuranose, to the coumarin‐based polyketide derived backbone. Expand
Enzymatic total synthesis of defucogilvocarcin M and its implications for gilvocarcin biosynthesis.
A complete, one-pot, enzymatic total synthesis of defucogilvocarcin M(1), a model compound that contains the unique chromophore common to all members of the gil Vocarcin group of natural products is reported. Expand
GilR, an Unusual Lactone‐Forming Enzyme Involved in Gilvocarcin Biosynthesis
It is shown that the enzyme, GilR, dehydrogenates the hemiacetal moiety of pregilvocarcin V to the lactone found in GV by using covalently bound FAD. Expand
Elucidation of post-PKS tailoring steps involved in landomycin biosynthesis.
The results revealed that LanM2 acts as a dehydratase and is responsible for concomitant release of the last PKS-tethered intermediate to yield prejadomycin (10), which was confirmed to be a general pathway intermediate of the biosynthesis. Expand