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Activation of the zymogen form of prostate-specific antigen by human glandular kallikrein 2.
Prostate-specific antigen (PSA) and human glandular kallikrein 2 (hK2) are glandular kallikreins secreted by the prostate gland. Both enzymes are synthesized with a propeptide that is supposedlyExpand
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Reporter genes lucFF, luxCDABE, gfp, and dsred have different characteristics in whole-cell bacterial sensors.
The selection of a genetic reporter can be difficult because of the wide range of genes available. In order to reduce the selection, we compared the performance of different reporter genes: fireflyExpand
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Bioluminescent yeast assays for detecting estrogenic and androgenic activity in different matrices.
In this paper we describe the construction and use of a set of bioluminescent yeast strains for the detection of compounds that can affect androgen or estrogen receptor mediated hormonal signalling.Expand
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Implication of an Outer Surface Lipoprotein in Adhesion of Bifidobacterium bifidum to Caco-2 Cells
ABSTRACT We found that the human intestinal isolate Bifidobacterium bifidum MIMBb75 strongly adhered to Caco-2 cells. Proteinase K and lithium chloride treatments showed that proteins play a key roleExpand
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A new recombinant cell-based bioluminescent assay for sensitive androgen-like compound detection.
A public concern is continuously arising about the presence of natural and anthropogenic compounds which affect human health by modulating normal endocrine functions. These substances, defined asExpand
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Recombinant luminescent bacteria for measuring bioavailable arsenite and antimonite.
Luminescent bacterial strains for the measurement of bioavailable arsenite and antimony were constructed. The expression of firefly luciferase was controlled by the regulatory unit of the ars operonExpand
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Measurement of bacterial adhesion-in vitro evaluation of different methods.
The adhesion of bacteria to host tissue is the first step in pathogenesis. Similarly, bacterial adhesion to inanimate surfaces is the first step in formation of biofilms-a real problem in industrialExpand
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A Luminescence-Based Mercury Biosensor
A new biosensor for the determination of bioavailable mercury is presented. It utilizes firefly luciferase gene as a reporter under the control of the mercury-inducible mer promoter from transposonExpand
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Oral Bacteria as Potential Probiotics for the Pharyngeal Mucosa
ABSTRACT The research described here was aimed at the selection of oral bacteria that displayed properties compatible with their potential use as probiotics for the pharyngeal mucosa. We included inExpand
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S-Layer Protein Mediates the Stimulatory Effect of Lactobacillus helveticus MIMLh5 on Innate Immunity
ABSTRACT The ability to positively affect host health through the modulation of the immune response is a feature of increasing importance in measuring the probiotic potential of a bacterial strain.Expand
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