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Purification, characterization and inhibition of dihydropyrimidinase from rat liver.
Dihydropyrimidinase (DHPase) was purified 564-fold over the initial rat liver extract, using heat, ammonium sulfate fractionation, DEAE-Sepharose CL-6B, carboxymethyl-Sepharose CL-6B, hydroxyapatiteExpand
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Purification, characterization and inhibition of D-3-aminoisobutyrate aminotransferase from the rat liver.
D-3-Aminoisobutyrate-pyruvate aminotransferase was purified 2000-fold from rat liver extract using heat treatment, ammonium sulfate fractionation, carboxylmethyl-Sepharose CL-6B, DEAE-SepharoseExpand
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Evaluation of interconversion between (R)- and (S)-enantiomers of β-aminoisobutyrate
Abstract The conversion of (R)- to (S)-β-aminoisobutyrate was observed in the presence of d -3-aminoisobutyrate-pyruvate aminotransferase, aminobutyrate aminotransferase, pyruvate and l -glutamate.Expand
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Effect of vitamin B2 deficiency on rat liver dihydropyrimidine dehydrogenase activity.
Effect of vitamin B2 deficiency on rat liver dihydropyrimidine dehydrogenase was investigated. It was found that the purified enzyme contains 2 mol flavin per molecule, which consists of equalExpand
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Molecular cloning and sequencing of a cDNA encoding dihydropyrimidinase from the rat liver.
A cDNA encoding dihydropyrimidinase has been isolated from a rat cDNA library. The N-terminal and an internal amino acid sequences were determined, and PCR primers were designed based on theseExpand
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Identity of D-3-aminoisobutyrate-pyruvate aminotransferase with alanine-glyoxylate aminotransferase 2.
D-3-Aminoisobutyrate-pyruvate aminotransferase (EC 2.6.1.40) and alanine-glyoxylate aminotransferase 2 (EC 2.6.1.44) were co-purified from rat liver as a single protein. The ratio of the twoExpand
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Cloning of rat dihydropyrimidine dehydrogenase and correlation of its mRNA increase in the rat liver with age.
Overlapping cDNAs encoding dihydropyrimidine dehydrogenase (DPD), the rate-limiting enzyme of pyrimidine degradation, were isolated from a rat liver cDNA library, and a 4,353 bp cDNA sequence with aExpand
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Analysis of cyclic feed intake in rats fed on a zinc-deficient diet and the level of dihydropyrimidinase (EC 3.5.2.2).
The body weight and feed intake of rats fed on a Zn-deficient diet for 28 d were reduced compared with those of control rats. The feed intakes of the Zn-deficient and control groups during the periodExpand
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Inhibition of d-3-aminoisobutyrate-pyruvate aminotransferase by 5-fluorouracil and α-fluoro-β-alanine
Among pyrimidine derivatives, we found that 5-fluorouracil;potently inhibited purified rat liver d -3-aminoisobutyrate-pyruvate aminotransferase, whereas 5-fluorouridine did so to a much lesserExpand
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Irreversible inhibition of D‐3‐aminoisobutyrate‐pyruvate aminotransferase by gabaculine
Gabaculine, 5‐amino‐l,3‐cyclohexadienylcarboxylate, is an analogue of GABA and a potent irreversible inhibitor of GABA aminotransferase. However, D‐3‐aminoisobutyrate‐pyruvate aminotransferase forExpand
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