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Purification, characterization and inhibition of dihydropyrimidinase from rat liver.
The results suggest that dihydropyrimidinase has no stereo specificities for 5-substituents of H2Ura, and the addition of N- carbamoyl-beta-alanine or N-carbamoyL- beta-amino-isobutyric acid showed hyperbolic mixed-type inhibition, when BrH2URA was used as the substrate.
Purification, characterization and inhibition of D-3-aminoisobutyrate aminotransferase from the rat liver.
- N. Tamaki, M. Kaneko, C. Mizota, M. Kikugawa, S. Fujimoto
- Biology, ChemistryEuropean journal of biochemistry
- 1 April 1990
6-Azauracil and 6-azathymine were found to be potent inhibitors of purified rat liver D-3-aminoisobutyrate-pyruvate aminotransferase, which catalyzed the transamination of omega-amino acids and pyruvic acid, glyoxylic acid and oxaloacetic acid were favorable amino acceptors.
Identity of D-3-aminoisobutyrate-pyruvate aminotransferase with alanine-glyoxylate aminotransferase 2.
Evaluation of interconversion between (R)- and (S)-enantiomers of β-aminoisobutyrate
Effect of vitamin B2 deficiency on rat liver dihydropyrimidine dehydrogenase activity.
- S. Fujimoto, K. Matsuda, M. Kikugawa, M. Kaneko, N. Tamaki
- BiologyJournal of nutritional science and vitaminology
- 1 February 1991
It was found that endogenous flavin may regulate the enzyme half-life or synthesis, and lowering of dihydropyrimidine dehydrogenase activity in the livers of rats fed on a vitamin B2-deficient diet did not affect the uridine, uracil and sigma UMP pool in liver.
Molecular cloning and sequencing of a cDNA encoding dihydropyrimidinase from the rat liver.
Cloning of rat dihydropyrimidine dehydrogenase and correlation of its mRNA increase in the rat liver with age.
- M. Kimura, S. Sakata, N. Tamaki
- Biology, ChemistryJournal of nutritional science and vitaminology
- 1 August 1998
Western blot analysis of COS-7 cells that were transfected with an RT-PCR-derived DPD cDNA showed that the product from the cDNA binds to an anti-serum for DPD, and the increase of DPD after birth may be caused by the increase in glucocorticoid level.
Analysis of cyclic feed intake in rats fed on a zinc-deficient diet and the level of dihydropyrimidinase (EC 22.214.171.124)
- N. Tamaki, S. Fujimoto-Sakata, M. Kikugawa, M. Kaneko, S. Onosaka, T. Takagi
- Biology, ChemistryBritish Journal of Nutrition
- 1 May 1995
Among pyrimidine-catabolizing enzymes, dihydropyrimidinase (EC3.5.2) activity showed significant retardation in the Zn-deficient rat liver with decrease of the enzyme protein.
Inhibition of d-3-aminoisobutyrate-pyruvate aminotransferase by 5-fluorouracil and α-fluoro-β-alanine