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The expanding world of methylotrophic metabolism.
TLDR
This chapter focuses on significant recent discoveries that are both refining and transforming the current understanding of methylotrophy as a metabolic phenomenon, and reviews new directions in methylotroph ecology that improve the understanding of the role of methylOTrophy in global biogeochemical processes.
Highly efficient methane biocatalysis revealed in a methanotrophic bacterium.
TLDR
It is demonstrated that methane assimilation is coupled with a highly efficient pyrophosphate-mediated glycolytic pathway, which under oxygen limitation participates in a novel form of fermentation-based methanotrophy, suggesting a novel mode of methane utilization in oxygen-limited environments.
Genetics of the glutamate‐mediated methylamine utilization pathway in the facultative methylotrophic beta‐proteobacterium Methyloversatilis universalis FAM5
TLDR
A gene cluster essential for operation of the N‐methylglutamate pathway in the methylotrophic beta‐proteobacterium Methyloversatilis universalis FAM5 is described and it is demonstrated that gltB‐like and sox‐like genes play a key role in methylamine utilization and encode N‐ methylglutamylmethylamide synthetase and N‐ethylglUTamate dehydrogenase respectively.
High-resolution metagenomics targets specific functional types in complex microbial communities
TLDR
A method to target microbial subpopulations by labeling DNA through stable isotope probing (SIP), followed by WGS sequencing, and extracting a nearly complete genome of a novel methylotroph, Methylotenera mobilis, reconstructing its metabolism and conducting genome-wide analyses is developed.
Classification of halo(alkali)philic and halo(alkali)tolerant methanotrophs provisionally assigned to the genera Methylomicrobium and Methylobacter and emended description of the genus
TLDR
Based on the results presented here and on previously reported morphological and physiological characteristics, these halotolerant and halophilic methanotrophic strains are classified as representing novel species within the genus Methylomicrobium.
Bacterial Populations Active in Metabolism of C1 Compounds in the Sediment of Lake Washington, a Freshwater Lake
TLDR
Overall, these data point toward the presence of a diverse population of active methylotrophs in Lake Washington sediments and toward the existence of yet-uncultivated organisms.
The enigmatic planctomycetes may hold a key to the origins of methanogenesis and methylotrophy.
TLDR
Phylogenetic analysis places the planctomycete sequences as distantly from their archaeal counterparts as from their proteobacterial counterparts, suggesting novel scenarios for the evolution of the C1 transfer functions in both methanogens and methylotrophs.
A metagenomic insight into freshwater methane-utilizing communities and evidence for cooperation between the Methylococcaceae and the Methylophilaceae
TLDR
The relevant abundances of the M methylococcaceae and the Methylophilaceae and their coordinated response to methane and nitrate suggest that these species may be engaged in cooperative behavior, the nature of which remains unknown.
Isolation and Characterization of Halotolerant Alkaliphilic Methanotrophic Bacteria from Tuva Soda Lakes
TLDR
The changes in cell phospholipids, fatty acids, and amino acids have been observed upon varying salinity and pH of the medium, thus suggesting structure-function osmoadaptation of the strains studied.
Characterization of a Novel Methanol Dehydrogenase in Representatives of Burkholderiales: Implications for Environmental Detection of Methylotrophy and Evidence for Convergent Evolution
TLDR
Novel, mono-subunit enzymes responsible for methanol oxidation in four strains are characterized, indicating that this ability is widespread beyond populations possessing mxaF, the gene traditionally used as a genetic marker for environmental detection of meetinghanol-oxidizing capability and suggesting a convergence toward the enzymatic function for meethanol oxide oxidation in MxaF and Mdh2-type proteins.
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