• Publications
  • Influence
Human complement C3b inactivator: isolation, characterization, and demonstration of an absolute requirement for the serum protein beta1H for cleavage of C3b and C4b in solution
The complement regulatory enzyme, C3b inactivator (C3bINA), has been purified from human serum by affinity chromatography on an anti-C3bINA Sepharose column. Subsequent chromatography onExpand
  • 628
  • 22
  • PDF
A Novel Sialic Acid Binding Site on Factor H Mediates Serum Resistance of Sialylated Neisseria gonorrhoeae
Factor H (fH), a key alternative complement pathway regulator, is a cofactor for factor I–mediated cleavage of C3b. fH consists of 20 short consensus repeat (SCR) domains. Sialic acid binding domainsExpand
  • 411
  • 19
  • PDF
Formation of the initial C3 convertase of the alternative complement pathway. Acquisition of C3b-like activities by spontaneous hydrolysis of the putative thioester in native C3
Activation of the alternative pathway of complement commences with the formation of an initial fluid-phase C3 convertase. Treatment of C3 with the nucleophilic reagent methylamine has previously beenExpand
  • 384
  • 13
  • PDF
Identification of three physically and functionally distinct binding sites for C3b in human complement factor H by deletion mutagenesis.
Human complement factor H controls spontaneous activation of complement in plasma and appears to play a role in distinguishing host cells from activators of the alternative pathway of complement. InExpand
  • 247
  • 13
Host recognition and target differentiation by factor H, a regulator of the alternative pathway of complement.
Factor H is responsible for recognition of host cells and tissues and mediates discrimination among microbial pathogens during activation of the alternative pathway of complement (AP). Its uniqueExpand
  • 176
  • 13
Complement C3 convertase: cell surface restriction of beta1H control and generation of restriction on neuraminidase-treated cells.
The alternative or properdin pathway of complement is primarily controlled by the endopeptidase C3b inactivator (C3bINA) and the nonproteolytic glycoprotein beta1H. The molecular mechanisms ofExpand
  • 310
  • 10
Complement control protein factor H: the good, the bad, and the inadequate.
The complement system is an essential component of the innate immune system that participates in elimination of pathogens and altered host cells and comprises an essential link between the innate andExpand
  • 259
  • 10
Binding of C4b-binding protein to porin: a molecular mechanism of serum resistance of Neisseria gonorrhoeae
We screened 29 strains of Neisseria gonorrhoeae and found 16/21 strains that resisted killing by normal human serum and 0/8 serum sensitive strains that bound the complement regulator, C4b-bindingExpand
  • 214
  • 10
  • PDF
Assembly and regulation of the membrane attack complex based on structures of C5b6 and sC5b9.
Activation of the complement system results in formation of membrane attack complexes (MACs), pores that disrupt lipid bilayers and lyse bacteria and other pathogens. Here, we present the crystalExpand
  • 133
  • 10
Analysis of the natural polymeric forms of human properdin and their functions in complement activation.
Many of the anomalies observed in studies or properdin may be explained on the basis of its ability to form a series of multi-subunit polymers and by differences in the functions of these forms ofExpand
  • 94
  • 9