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IscU as a scaffold for iron-sulfur cluster biosynthesis: sequential assembly of [2Fe-2S] and [4Fe-4S] clusters in IscU.
The ability to assemble both [2Fe-2S](2+) and [4Fe-4S]-2+) clusters in IscU supports the proposal that this ubiquitous protein provides a scaffold for IscS-mediated assembly of clusters that are subsequently used for maturation of apo Fe-S proteins.
NifS-directed assembly of a transient [2Fe-2S] cluster within the NifU protein.
- P. Yuvaniyama, J. Agar, V. Cash, M. Johnson, D. Dean
- Chemistry, BiologyProceedings of the National Academy of Sciences…
- 18 January 2000
NifU is able to interact with NifS and that a second, transient [2Fe-2S] cluster can be assembled within NifU in vitro when incubated in the presence of ferric ion, L-cysteine, and catalytic amounts of NIFS.
Improved assay of neurotoxic esterase for screening organophosphates for delayed neurotoxicity potential
- M. Johnson
- Chemistry, MedicineArchives of Toxicology
- 18 June 1977
The assay of neurotoxic esterase (NTE) in brains taken from dosed hens enables potential neurotoxicity of organophosphate pesticides, plasticers, etc. to be assessed. The original assay [Johnson, M.…
IscA, an alternate scaffold for Fe-S cluster biosynthesis.
It is proposed that the IscA family of proteins provide alternative scaffolds to the NifU and IscU proteins for mediating nif-specific and general Fe-S cluster assembly.
Effects of Salts on the Halophilic Alga Dunaliella viridis
- M. Johnson, E. Johnson, R. MacElroy, H. L. Speer, B. Bruff
- Biology, ChemistryJournal of Bacteriology
- 1 April 1968
Determinations of the salt sensitivity of enzymes extracted from the halophilic alga Dunaliella viridis revealed that pentose phosphate isomerase, ribulose diphosphate carboxylase,…
The role and properties of the iron-sulfur cluster in Escherichia coli dihydroxy-acid dehydratase.
Effects of elemental sulfur on the metabolism of the deep-sea hyperthermophilic archaeon Thermococcus strain ES-1: characterization of a sulfur-regulated, non-heme iron alcohol dehydrogenase
It is proposed that, under So limitation, ADH reduces to alcohols the aldehydes that are generated by fermentation, thereby serving to dispose of excess reductant.
The intracellular distribution of glycolytic and other enzymes in rat-brain homogenates and mitochondrial preparations.
- M. Johnson
- BiologyBiochemical Journal
- 1 December 1960
Modular organization and identification of a mononuclear iron-binding site within the NifU protein
- J. Agar, P. Yuvaniyama, M. Johnson
- Chemistry, BiologyJBIC Journal of Biological Inorganic Chemistry
- 1 April 2000
A model where iron bound in labile rubredoxin-like sites within NifU is used for [Fe-S] cluster formation is provided, and primary sequence comparisons, amino acid substitution experiments, and optical and resonance Raman spectroscopic characterization of recombinantly produced NIFU and Nif U fragments are used to show that Nifu has a modular structure.
The delayed neurotoxic effect of some organophosphorus compounds. Identification of the phosphorylation site as an esterase.
- M. Johnson
- Biology, ChemistryBiochemical Journal
- 1 October 1969
A small proportion of the total activity of hen brain hydrolysing phenyl phenylacetate in vitro was shown to be due to an enzyme different from two others previously described, and it was deduced that the active site is the phosphorylation site associated with the genesis of delayed neurotoxicity.