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Proteolysis of SNAP‐25 Isoforms by Botulinum Neurotoxin Types A, C, and E
Abstract : Tetanus toxin and the seven serologically distinct botulinal neurotoxins (BoNT/A to BoNT/G) abrogate synaptic transmission at nerve endings through the action of their light chains (LExpand
Light-induced flickering of DsRed provides evidence for distinct and interconvertible fluorescent states.
Green fluorescent protein (GFP) from jellyfish Aequorea victoria, the powerful genetically encoded tag presently available in a variety of mutants featuring blue to yellow emission, has found aExpand
Triple FRET: a tool for studying long-range molecular interactions.
An evolved aminoacyl-tRNA synthetase with atypical polysubstrate specificity.
We have employed a rapid fluorescence-based screen to assess the polyspecificity of several aminoacyl-tRNA synthetases (aaRSs) against an array of unnatural amino acids. We discovered that aExpand
Site-specific labeling of proteins with NMR-active unnatural amino acids
A large number of amino acids other than the canonical amino acids can now be easily incorporated in vivo into proteins at genetically encoded positions. The technology requires an orthogonalExpand
Outwitting EF-Tu and the ribosome: translation with d-amino acids
Key components of the translational apparatus, i.e. ribosomes, elongation factor EF-Tu and most aminoacyl-tRNA synthetases, are stereoselective and prevent incorporation of d-amino acids (d-aa) intoExpand
Accessing Molecular Dynamics in Cells by Fluorescence Correlation Spectroscopy
Abstract Fluorescence correlation spectroscopy (FCS) analyzes spontaneous fluctuations in the fluorescence emission of small molecular ensembles, thus providing information about a multitude ofExpand
A thermostable d-polymerase for mirror-image PCR
Abstract Biological evolution resulted in a homochiral world in which nucleic acids consist exclusively of d-nucleotides and proteins made by ribosomal translation of l-amino acids. From theExpand
A New Embedded Process for Compartmentalized Cell‐Free Protein Expression and On‐line Detection in Microfluidic Devices
During the last century, an enormous number of protein functions were identified, many of which can be generally described as catalytic. Ongoing research currently focuses on two aspects, analysis ofExpand
Triple-color coincidence analysis: one step further in following higher order molecular complex formation.
Confocal fluorescence spectroscopy is a versatile method for studying dynamics and interactions of biomolecules in their native environment with minimal interference with the observed system.Expand