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Optimisation of transformation conditions and production of transgenic sorghum (Sorghum bicolor) via microparticle bombardment
AbstractConditions for microparticle bombardment were optimised for four explant types of sorghum Sorghum bicolor (L.) Moench based on transient expression of the uidA reporter gene. The testedExpand
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In vitro culture of the sugarbeet: Description of a cell line with high regeneration capacity
Abstract Leaf pieces of sugarbeet plants were incubated on different media. The primary calluses were subcultured on different hormone combinations and subjected to cold treatment during 3–9 weeks.Expand
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Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna obtained by “gamma-fusion”
SummaryAsymmetric nuclear hybrids have been obtained by fusion of cells from a nitrate-reductase deficient mutant of Nicotiana plumbaginifolia (cnx20) and gamma irradiated protoplasts of AtropaExpand
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Overexpression of ornithine-δ-aminotransferase increases proline biosynthesis and confers osmotolerance in transgenic plants
Accumulationof proline is a way to increase tolerance to water stress in plants. Therefore,considerable attention has been devoted to optimise proline biosynthesis intransgenic plants. Glutamate andExpand
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Dihydrodipicolinate synthase ofnicotiana sylvestris, a chloroplast-localized enzyme of the lysine pathway
The first enzyme of the lysine-biosynthesis pathway, dihydrodipicolinate synthase (DHDPS; EC 4.2.1.52) has been purified and characterized inNicotiana sylvestris Speggazini et Comes. A purificationExpand
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Threonine Accumulation in a Mutant of Arabidopsis thaliana (L.) Heynh. with an Altered Aspartate Kinase
Summary After mutagenesis, M 2 seedlings of Arabidopsis thaliana were grown on a selective medium containing toxic concentrations of lysine and threonine. One of the LT-resistant mutants (RLT 40) wasExpand
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The Arabidopsis thaliana dhdps gene encoding dihydrodipicolinate synthase, key enzyme of lysine biosynthesis, is expressed in a cell-specific manner
Lysine synthesis in prokaryotes, some phycomycetes and higher plants starts with the condensation of L-aspartate-β-semialdehyde (L-ASA) and pyruvate into dihydrodipicolinic acid. The enzyme thatExpand
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The art of colour
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Sequence analysis, transcriptional and posttranscriptional regulation of the rice vdac family.
The voltage-dependent anion-selective channel (VDAC) is a mitochondrial outer membrane ion channel. Different isoforms exist in plants but information about their specific role remains to beExpand
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Lysine overproducer mutants with an altered dihydrodipicolinate synthase from protoplast culture of Nicotiana sylvestris (Spegazzini and Comes)
SummaryTwo S-(2-aminoethyl)L-cysteine (AEC) resistant lines were isolated by screening mutagenized protoplasts from diploid N. sylvestris plants. Both lines accumulated free lysine at levels 10 toExpand
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