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Crystal Structure of a Bacterial Type III Polyketide Synthase and Enzymatic Control of Reactive Polyketide Intermediates*
TLDR
The crystal structure of Streptomyces coelicolor THNS reveals an unanticipated novel cavity extending into the “floor” of the traditional active site cavity, providing the first plausible structural and mechanistic explanation for yet another unusual THNS catalytic activity: its previously inexplicable extra polyketide extension step when primed with a long acyl starter. Expand
Isolation of azaspiracid-2 from a marine sponge Echinoclathria sp. as a potent cytotoxin.
TLDR
A combination of HPLC using ODS, GS320, and Phenylhexyl stationary phases permitted the purification without using acid or inorganic additives in the mobile phase of Azaspiracid-2 to exhibit potent cytotoxicity against P388 cells. Expand
Engineered Streptomyces avermitilis host for heterologous expression of biosynthetic gene cluster for secondary metabolites.
TLDR
Production of metabolites in some transformants of the versatile host was higher than that of the original producers, and cryptic biosynthetic gene clusters in the original producer were also expressed in a versatile host. Expand
Class of cyclic ribosomal peptide synthetic genes in filamentous fungi.
TLDR
Two function-unknown genes (ustYa/ustYb) in the gene cluster for ustiloxins from Aspergillus flavus were found experimentally to be involved in cyclization of the peptide. Expand
Expression and characterization of the type III polyketide synthase 1,3,6,8-tetrahydroxynaphthalene synthase from Streptomyces coelicolor A3(2)
TLDR
Incubation of recombinant THNS with malonyl-CoA showed THN production, as demonstrated by UV and HPLC analyses, and the inactivation, expression, and characterization of the type III PKS homologous SCO1206 gene product as 1,3,6,8-tetrahydroxynaphthalene synthase (THNS) was reported. Expand
Novel thioviridamide derivative—JBIR-140: heterologous expression of the gene cluster for thioviridamide biosynthesis
Novel thioviridamide derivative—JBIR-140: heterologous expression of the gene cluster for thioviridamide biosynthesis
Mutasynthesis of enterocin and wailupemycin analogues.
TLDR
The strict starter unit specificity observed in vivo indicates that the enterocin type II polyketide synthase (PKS) exerts selective control over the choice of starter units. Expand
Cloning of modular type I polyketide synthase genes from salinomycin producing strain of Streptomyces albus.
TLDR
Cloning of polyether polyketide synthase (PKS) genes for salinomycin biosynthetic gene cluster of Streptomyces albus showed high homology with bacterial type I PKSs and was deduced to code for KS, malonyl transferase, and ketoreductase motifs. Expand
JBIR-94 and JBIR-125, antioxidative phenolic compounds from Streptomyces sp. R56-07.
TLDR
Two phenolic compounds were isolated from the fermentation broth of strain R56-07, which was identified by phylogenetic methods as a novel species of Streptomyces, and are the first examples of hydroxycinnamic acid amides containing putrescine or spermidine produced by actinomycetes. Expand
Identification and Characterization of the Streptazone E Biosynthetic Gene Cluster in Streptomyces sp. MSC090213JE08
TLDR
A pathway for the biosynthesis of streptazone E is proposed in which the two putative cyclases of the nuclear transport factor 2–like superfamily are responsible for C−C bond formation coupled with epoxide ring opening to give the five‐membered ring of strePTazone  E. Expand
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