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Optimized green fluorescent protein fused to FoF1-ATP synthase for single-molecule FRET using a fast anti-Brownian electrokinetic trap
TLDR
This ABELtrap with a laser focus pattern generated by electro-optical beam deflectors and controlled by a programmable FPGA chip increases the observation time of fluorescent nanoparticles compared to free diffusion by a factor of 10000, and monitors conformational changes of individual membrane proteins in real time.
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Imaging cytochrome C oxidase and FoF1-ATP synthase in mitochondrial cristae of living human cells by FLIM and superresolution microscopy
TLDR
Their mitochondrial nano-environment was investigated by FLIM and superresolution microscopy in living human cells and different lifetimes and anisotropy values were found.
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USE OF DIFFERENTIAL LYSIS FOR DNA ISOLATION TO CONFIRM SPERM TRANSFECTION
TLDR
A method for confirming the transfection of sperm with plasmid DNA with the specificity of the PCR products was developed and a pair of oligonucleotide primers for the amplification of pET-28c DNA was obtained.
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The Models of Organization of Accounting with Use of Crowd Technologies: Features, Advantages, Prospects
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EXPERIMENTAL STUDY OF EXOTERMIC EFFECTS INSIDE DENTAL PULP CHAMBER WHEN APPLYING DIRECT TECHNIQUE OF MANUFACTURING TEMPORARY CROWN
  • M. IlchenkoN. Dydyk
  • Materials Science, Medicine
    Актуальні проблеми сучасної медицини: Вісник Української медичної стоматологічної академії
  • 17 June 2021
TLDR
Exothermic effects during intra oral fabrication of temporary crowns can be minimized by polymerization of resins in the silicone mould as this material can absorb and dissipate heat.
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