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Lipocalin 2 mediates an innate immune response to bacterial infection by sequestrating iron
TLDR
This finding represents a new component of the innate immune system and the acute phase response to infection and limits bacterial growth by sequestrating the iron-laden siderophore. Expand
The neutrophil lipocalin NGAL is a bacteriostatic agent that interferes with siderophore-mediated iron acquisition.
TLDR
It is proposed that NGAL participates in the antibacterial iron depletion strategy of the innate immune system by tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions. Expand
Crystal structure of a γδ T-cell receptor specific for the human MHC class I homolog MICA
TLDR
Interaction analyses showed that MIC binding by the two receptors was mutually exclusive, and analysis of relative binding kinetics suggested sequential recognition, defining constraints for the temporal organization of γδ T-cell/target cell interfaces. Expand
HLA-E Allelic Variants
TLDR
Compared expression levels, and studies of the physical and biochemical properties of these two proteins, it was found that whereas steady-state protein levels were similar, the two alleles did in fact differ with respect to cell surface levels, which may help explain the apparent balancing selection acting on this locus. Expand
Computational design of epitope-scaffolds allows induction of antibodies specific for a poorly immunogenic HIV vaccine epitope.
TLDR
This work describes a general computational method to design epitope-scaffolds in which contiguous structural epitopes are transplanted to scaffold proteins for conformational stabilization and immune presentation and demonstrates that computationally designed epitopes-scafolds are valuable as structure-specific serological reagents and as immunogens to elicit antibodies with predetermined structural specificity. Expand
CORRELATING DIFFERENTIAL EXPRESSION, PEPTIDE AFFINITIES, CRYSTAL STRUCTURES, AND THERMAL STABILITIES*
TLDR
Compared expression levels, and studies of the physical and biochemical properties of these two proteins, it was found that whereas steady-state protein levels were similar, the two alleles did in fact differ with respect to cell surface levels, which may help explain the apparent balancing selection acting on this locus. Expand
Structure of thermolysin refined at 1.6 A resolution.
TLDR
The structure of the thermostable protease thermolysin has been refined by a restrained least-squares procedure and supports the previous assertion that the ther mostable proteins in general is due to a combination of factors which can include hydrophobic interactions, hydrogen bonding, ionic interactions, disulfide linkages, metal binding and other forms of stabilization. Expand
Interactions between Lipids and Human Anti-HIV Antibody 4E10 Can Be Reduced without Ablating Neutralizing Activity
TLDR
Results show that 4E10 nonspecific membrane binding is separable from neutralization, which is achieved through specific peptide/lipid orientation changes. Expand
“Superhumanized” Antibodies: Reduction of Immunogenic Potential by Complementarity-Determining Region Grafting with Human Germline Sequences: Application to an Anti-CD281
TLDR
A new approach to humanizing murine mAbs that may reduce immunogenicity even further is reported and the humanized 9.3 showed biological activity as an immunosuppressant by inhibiting a MLR. Expand
Siderocalin (Lcn 2) also binds carboxymycobactins, potentially defending against mycobacterial infections through iron sequestration.
TLDR
It is shown that siderocalin also binds soluble siderophores of mycobacteria, including M. tuberculosis: carboxymycobactins, and employs a degenerate recognition mechanism to cross react with these dissimilar types of siders, broadening the potential utility of this innate immune defense. Expand
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