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Dual epitope recognition by the VASP EVH1 domain modulates polyproline ligand specificity and binding affinity
It is proposed that this epitope, which is absent in the sequences of the native EVH1‐interaction partners zyxin and vinculin, may provide the pathogen with an advantage when competing for the recruitment of the host VASP and Mena proteins in the infected cell. Expand
How To Determine Diffusion Coefficients in Planar Phospholipid Systems by Confocal Fluorescence Correlation Spectroscopy
Confocal fluorescence correlation spectroscopy (FCS) allows for the determination of lateral diffusion coefficients and surface densities in planar phospholipid systems. The determination of theExpand
Effects of alkali cations and halide anions on the DOPC lipid membrane.
The simulations revealed that sodium is attracted to the headgroup region with its concentration being maximal in the vicinity of the phosphate groups, this effect being likely underestimated within the present nonpolarizable force field. Expand
Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions.
The lateral diffusion coefficients of a BODIPY tail-labeled lipid in two model systems, namely, free-standing giant unilamellar vesicles (GUVs) and supported phospholipid bilayers (SPBs), wereExpand
Headgroup hydration and mobility of DOTAP/DOPC bilayers: a fluorescence solvent relaxation study.
The fluorescence solvent relaxation technique is applied to study the structure and dynamics of fully hydrated liquid crystalline lipid bilayers composed of mixtures of cationic dioleoyltrimethylammoniumpropane (DOTAP) and neutral dioeloylphosphatidylcholine (DOPC). Expand
Fluorescence Lifetime Correlation Spectroscopy
The basic principles of FLCS are explained, a genuine fusion of Time-Correlated Single Photon Counting and Fluorescence Correlation Spectroscopy, using common terms and minimum mathematics. Expand
Molecular rheometry: direct determination of viscosity in Lo and Ld lipid phases via fluorescence lifetime imaging.
This study showcases the power of FLIM with molecular rotors to image microviscosity of heterogeneous microenvironments in complex biological systems, including membrane-localised lipid rafts. Expand
Structure, dynamics, and hydration of POPC/POPS bilayers suspended in NaCl, KCl, and CsCl solutions.
The experimental findings are supported by molecular dynamics simulations, which show deep penetration of the cations down to the glycerol level of the lipid bilayer where they pair with oxygen atoms of carbonyl groups and follow the same Hofmeister ordering as the cationic adsorption to the bilayer. Expand
Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy.
This work discusses several key questions of lateral mobility investigation in planar lipid membranes, namely the influence which membrane and aqueous phase composition, choice of a fluorescent tracer molecule, frictional coupling between the two membrane leaflets and between membrane and solid support has on the lateral mobility of lipids. Expand
Biophysics of lipid bilayers containing oxidatively modified phospholipids: insights from fluorescence and EPR experiments and from MD simulations.
It was shown that the presence of the investigated oxPCs in phospholipid model membranes may have the following consequences: decrease of the lipid order, lowering of phase transition temperatures, lateral expansion and thinning of the bilayer, alterations of bilayer hydration profiles, increased lipid mobility, and augmented flip-flop. Expand