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A practical guide to the MaxQuant computational platform for SILAC-based quantitative proteomics
MaxQuant is a quantitative proteomics software package designed for analyzing large mass spectrometric data sets. Expand
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Phosphatidylcholine synthesis for lipid droplet expansion is mediated by localized activation of CTP:phosphocholine cytidylyltransferase.
Lipid droplets (LDs) are cellular storage organelles for neutral lipids that vary in size and abundance according to cellular needs. Physiological conditions that promote lipid storage rapidly andExpand
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Triple SILAC to Determine Stimulus Specific Interactions in the Wnt Pathway
Many important regulatory functions are performed by dynamic multiprotein complexes that adapt their composition and activity in response to different stimuli. Here we employ quantitative affinityExpand
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Structure-Based Prediction of Asparagine and Aspartate Degradation Sites in Antibody Variable Regions
Monoclonal antibodies (mAbs) and proteins containing antibody domains are the most prevalent class of biotherapeutics in diverse indication areas. Today, established techniques such as immunizationExpand
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Protein Correlation Profiles Identify Lipid Droplet Proteins with High Confidence*
Lipid droplets (LDs) are important organelles in energy metabolism and lipid storage. Their cores are composed of neutral lipids that form a hydrophobic phase and are surrounded by a phospholipidExpand
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Systems-wide Analysis of a Phosphatase Knock-down by Quantitative Proteomics and Phosphoproteomics
Signal transduction in metazoans regulates almost all aspects of biological function, and aberrant signaling is involved in many diseases. Perturbations in phosphorylation-based signaling networksExpand
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Phosphorylation of SUMO-1 occurs in vivo and is conserved through evolution.
Protein dynamics is regulated by an elaborate interplay between different post-translational modifications. Ubiquitin and ubiquitin-like proteins (Ubls) are small proteins that are covalentlyExpand
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A Two-pronged Binding Mechanism of IgG to the Neonatal Fc Receptor Controls Complex Stability and IgG Serum Half-life*
The success of recombinant monoclonal immunoglobulins (IgG) is rooted in their ability to target distinct antigens with high affinity combined with an extraordinarily long serum half-life, typicallyExpand
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Investigating the Interaction between the Neonatal Fc Receptor and Monoclonal Antibody Variants by Hydrogen/Deuterium Exchange Mass Spectrometry*
The recycling of immunoglobulins by the neonatal Fc receptor (FcRn) is of crucial importance in the maintenance of antibody levels in plasma and is responsible for the long half-lives of endogenousExpand
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Molecular polygamy: The promiscuity of l‐phenylalanyl‐tRNA‐synthetase triggers misincorporation of meta‐ and ortho‐tyrosine in monoclonal antibodies expressed by Chinese hamster ovary cells
In‐depth analytical characterization of biotherapeutics originating from different production batches is mandatory to ensure product safety and consistent molecule efficacy. Previously, we have shownExpand
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