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Dissection of the ATP-induced conformational cycle of the molecular chaperone Hsp90
This comprehensive reconstitution of the Hsp90 cycle defines a controlled progression through distinct intermediates that can be modulated by conformation-sensitive cochaperones.
Asymmetric activation of the hsp90 dimer by its cochaperone aha1.
The mechanism of the Aha1-mediated acceleration of Hsp90 ATPase activity and the interaction surfaces of both proteins using multidimensional NMR techniques are analyzed and it is found that one subunit can be used for conformational ATPase regulation and the other for substrate protein processing.
The large conformational changes of Hsp90 are only weakly coupled to ATP hydrolysis
A single-molecule assay that allows examination of yeast Hsp90 dimers in real time under various nucleotide conditions is developed and conformational fluctuations between open and closed states are detected on timescales much faster than the rate of ATP hydrolysis.
Noncatalytic Role of the FKBP52 Peptidyl-Prolyl Isomerase Domain in the Regulation of Steroid Hormone Signaling
The results suggest that the FK1 domain, and in particular the loop overhanging the catalytic pocket, is critically involved in receptor interactions and receptor activity.
Conserved Conformational Changes in the ATPase Cycle of Human Hsp90*
The results show that the basic principles of the Hsp90 ATPase reaction are conserved between yeast and humans, including the dimerization of the N-terminal domains and its regulation by the repositioning of the ATP lid from its original position to a catalytically competent one.
Ultraviolet irradiation doses for coronavirus inactivation – review and analysis of coronavirus photoinactivation studies
Since coronaviruses do not differ structurally to any great exent, the SARS-CoV-2 virus – as well as possible future mutations – will very likely be highly UV sensitive, so that common UV disinfection procedures will inactivate the new SARS-CoVs without any further modification.
A fully synthetic human Fab antibody library based on fixed VH/VL framework pairings with favorable biophysical properties
It is confirmed that quality can be built into an antibody library by prudent selection of unmodified, fully human VH/VL pairs as scaffolds by means of a systematic analysis of a large set of rearranged human antibody sequences.
The measurement of tropospheric OH radicals by laser‐induced fluorescence spectroscopy during the POPCORN Field Campaign
A highly sensitive OH measurement instrument has been developed. It is based on laser-induced fluorescence (LIF) detection of OH using the A²Σ+v′ = 0-X²Π v″ = 0 transition at 308.15 nm at low
In situ measurement of tropospheric OH radicals by laser-induced fluorescence - a description of the KFA instrument
Abstract An instrument for the measurement of tropospheric OH radical concentrations by laser-induced fluorescence spectroscopy has been developed. Ambient air is expanded through a nozzle into a
Hsp90 charged-linker truncation reverses the functional consequences of weakened hydrophobic contacts in the N domain
Data underscore the importance of β-strand 8 for Hsp90 function and demonstrate that the functional consequences of weakened hydrophobic contacts in this region are reversed by charged-linker truncation.