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Allopolyploidy in Wheat Induces Rapid and Heritable Alterations in DNA Methylation Patterns of Cellular Genes and Mobile Elements
Global DNA fingerprinting of a newly synthesized allohexaploid wheat and its natural counterpart, the common wheat, by AFLP analysis showed that the main type of changes in the amphiploid is epigenetic in nature, i.e., alteration in DNA methylation patterns. Expand
Somatic embryogenesis and its hormonal regulation in tissue cultures of Freesia refracta.
Explants of F. refracta cultured in vitro can be induced to undergo somatic embryogenesis via two different pathways, i.e. directly from epidermal cell of the explants, or indirectly through anExpand
Protoplast culture and plant regeneration ofPinellia ternata
K8P and modified MS media were used to culture protoplasts in: a) liquid, b) liquid-solid double layer, or c) agarose embedded protoplast culture, and the former two were conducive to colony formation fromprotoplast-derived cells. Expand
Chromosome variation of pollen mother cell of pollen-derived plants in wheat (Triticum aestivum L.).
It is suggested that new types of chromosome variant plants may be obtained by means of anther culture method and several nullisomical, octoploidy and mixoploids plants set seeds in about 10% of heteroploids and aneuploids obtained by us. Expand
The Ultrastructural Evidence on the Origin of Protein Bodies in the Rough Endoplasmic Reticulum of Developing Cotyledons of Soybean
The direct development of protein bodies from rough endoplasmic reticulum at the late stages of soybean seed development is reported and some structures which have not been reported before are observed and described. Expand
Microdissection of chromosome 7B of common wheat and cloning of low-copy specific DNA sequences
Genomic Southern hybridization confirmed that the PCR products were originated from the wheat genome and demonstrated that all the 6 low-copy clones were specific to either chromosome 78 or the 7th homoeologous group, whereas the 3 arbitrarily chosen repetitive clones were non-specific, disperse sequences. Expand
Cell culture induced introgression of Thinopyrum intermedium chromatin into common wheat
To introduce useful genes from the wild species Thinopyrum intermedium into cultivated wheat, a wheat-Th. intermedium disomic addition line (TAI27) was used as source material for tissue culture.Expand
Construction of the seed-coat cDNA microarray and screening of differentially expressed genes in barley.
It was found that most of these overexpressing clones were transcription/translation and hordein-associated genes, which have laid a solid material basis for further elucidation of the metabolic pathway in proanthocyanidin synthesis in barley and likely other plants. Expand
Configuration of nucleolar DNAin situ in nucleolus ofAllium cepa cells
The nucleolar ultrastructure and the character of nucleolar DNA inAllium cepa cells are observed by conventional electron microscopy and the cytochemical NAMA-Ur DNA specific staining method and it is indicated that the nucleolarDNA in allium cEPa cells is mainly located at the border between fibrillar centers and dense fibrilar component. Expand
Variation of wheatgrass chromosomes in wheat-wheatgrass alien addition line “TAI-27” revealed by fluorescencein situ hybridization (FISH)
  • F. Han, X. Zhang, +4 authors Z. Xin
  • Biology, Medicine
  • Science in China Series C: Life Sciences
  • 1 August 1998
FISH technique was used to analyze the two morphologically different plants, and observations indicate that a pair of small chromosomes in one varietion line are from wheatgrass and another pair of wheatgrass chromosomes have substituted the wheat chromosomes. Expand