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TRIM5 is an innate immune sensor for the retrovirus capsid lattice
TLDR
The retroviral restriction factor TRIM5 has two additional activities that are linked to restriction: it constitutively promotes innate immune signalling and it acts as a pattern recognition receptor specific for the retrovirus capsid lattice. Expand
Drug Export Pathway of Multidrug Exporter AcrB Revealed by DARPin Inhibitors
TLDR
The structure of the trimeric AcrB in complex with a designed ankyrin-repeat protein (DARPin) inhibitor suggests a transport pathway not through the central pore but through the identified channels in the individual subunits, which greatly advances the understanding of the multidrug export mechanism. Expand
Transport of drugs by the multidrug transporter AcrB involves an access and a deep binding pocket that are separated by a switch-loop
TLDR
High resolution structures are reported of AcrB/designed ankyrin repeat protein (DARPin) complexes with bound minocycline or doxorubicin and transport seems to be a stepwise process of initial drug uptake in the access pocket of the L monomer and subsequent accommodation of the drug in the deep binding pocket during the L to T transition. Expand
Structural basis of chaperone–subunit complex recognition by the type 1 pilus assembly platform FimD
TLDR
Nuclear magnetic resonance and X‐ray protein structures of the N‐terminal substrate recognition domain of FimD (FimDN) before and after binding of a chaperone–subunit complex are reported and it is shown how this mechanism enables recognition and discrimination of different chaper one–sub unit complexes by bacterial pilus assembly platforms. Expand
Crystal structure of a heterodimeric ABC transporter in its inward-facing conformation
TLDR
The 2.9-Å crystal structure of the heterodimeric ABC transporter TM287–TM288 (TM287/288) from Thermotoga maritima provides unprecedented insights into the mechanism of heterodenderic ABC exporters and will enable future studies on this large transporter superfamily. Expand
Crystal structure of the multidrug exporter MexB from Pseudomonas aeruginosa.
TLDR
The X-ray structure of MexB completes the molecular picture of the major pump mediating intrinsic and acquired multidrug resistance in P. aeruginosa. Expand
Structure-based design of aliskiren, a novel orally effective renin inhibitor.
TLDR
Aliskiren is the first in a novel class of renin inhibitors with the potential for treatment of hypertension and related cardiovascular diseases and employs a combination of molecular modelling and crystallographic structure analysis to design renin inhibitor lacking the extended peptide-like backbone of earlier inhibitors. Expand
The Long Form of FLIP Is an Activator of Caspase-8 at the Fas Death-inducing Signaling Complex*
TLDR
It is proposed that FLIPL inhibits caspase-8 release-dependent pro-apoptotic signals, whereas the single, membrane-restricted active site of theFLIPL-casp enzyme-inhibitory protein long form heterocomplex is proteolytically active and acts on local substrates such as RIP. Expand
Crystal structure and functional analysis of Escherichia coli glutamate decarboxylase
TLDR
It is demonstrated that the enzyme is localized exclusively in the cytoplasm at neutral pH, but is recruited to the membrane when the pH falls, and the triple helix bundle formed by the N‐termini of the protein at acidic pH is the major determinant for this behaviour. Expand
High-affinity binders selected from designed ankyrin repeat protein libraries
TLDR
The evolution of ankyrin repeat (AR) proteins in vitro for specific, high-affinity target binding and combinatorial libraries of AR proteins of varying repeat numbers with diversified binding surfaces are generated, an attractive alternative to antibody libraries. Expand
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