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The crystal structures of native and (S)-lysine-bound dihydrodipicolinate synthase from Escherichia coli with improved resolution show new features of biological significance.
TLDR
The general architecture of the active site is found to be consistent with previously determined structures, but with some important differences, and a re-evaluation of the dimer-dimer interface reveals a more extensive network of interactions than first thought. Expand
Dihydrodipicolinate synthase (DHDPS) from Escherichia coli displays partial mixed inhibition with respect to its first substrate, pyruvate.
TLDR
The inhibition mechanism of DHDPS with respect to (S)-lysine was re-evaluated by incorporating the observed hyperbolic inhibition, showing that lysine is not an uncompetitive inhibitor, but a mixed inhibitor when pyruvate and (S-lysine concentrations were varied. Expand
Conformational switching of the pseudokinase domain promotes human MLKL tetramerization and cell death by necroptosis
TLDR
Evidence is provided that the transition of human MLKL from a monomeric state to a tetramer is essential for necroptosis signalling. Expand
The structure of human interleukin-11 reveals receptor-binding site features and structural differences from interleukin-6.
TLDR
Comparisons show important differences between the two cytokines and it is suggested that IL-11 engages GP130 differently to IL-6, and these data offer insight into the binding interactions ofIL-11 with each of its receptors and the structural mechanisms underlying agonist and antagonist variants of the protein. Expand
Small Oligomers of Ribulose-bisphosphate Carboxylase/Oxygenase (Rubisco) Activase Are Required for Biological Activity
TLDR
A variety of techniques are used to show that Rubisco activase forms a wide range of structures in solution, ranging from monomers to much higher order species, and that the distribution of these species is highly dependent on protein concentration. Expand
Evolution of quaternary structure in a homotetrameric enzyme.
TLDR
It is proposed that the homotetrameric structure of DHDPS reduces dynamic fluctuations present in the dimeric forms and increases specificity for the first substrate, pyruvate, and small-angle X-ray scattering and mutagenesis data support this hypothesis and lead to the suggestion that in at least some cases the evolution of quaternary enzyme structures might serve to optimise the dynamic properties of the protein subunits. Expand
Methionine oxidation induces amyloid fibril formation by full-length apolipoprotein A-I
TLDR
This systematic study of fibrillar formation by full-length apoA-I represents the first demonstration that methionine oxidation can induce amyloid fibril formation. Expand
Transferrin receptor 1 is a reticulocyte-specific receptor for Plasmodium vivax
TLDR
Using Brazilian and Thai clinical isolates, data show that TfR1-PvRBP2b invasion pathway is critical for the recognition of reticulocytes during P. vivax invasion. Expand
Avoiding the oligomeric state: αB‐crystallin inhibits fragmentation and induces dissociation of apolipoprotein C‐II amyloid fibrils
TLDR
Previously unrecognized mechanisms of αB‐c chaperone action in amyloid assembly and fibril dynamics are revealed, and a rationale for the in vivo colocalization of small heat‐shock proteins with amyloids deposits is provided. Expand
Notch ligand delta-like1: X-ray crystal structure and binding affinity.
TLDR
The X-ray crystal structure of the extracellular domain of the Notch ligand delta-like ligand-1 (Dll-1) is determined, which incorporates the N-terminal C2 domain, receptor-binding DSL domain and the first six EGF-like repeats, which form a highly extended conformation, confirmed by analytical ultracentrifugation. Expand
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