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Integration Site for Streptomyces Phage φBT1 and Development of Site-Specific Integrating Vectors
- M. Gregory, R. Till, Margaret C. M. Smith
- Biology, EngineeringJournal of bacteriology
- 1 September 2003
It is demonstrated that phiBT1 integrates into a different attachment site than phiC31, which lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein.
Biosynthesis of the immunosuppressants FK506, FK520, and rapamycin involves a previously undescribed family of enzymes acting on chorismate
- J. Andexer, S. Kendrew, B. Wilkinson
- Biology, ChemistryProceedings of the National Academy of Sciences
- 7 March 2011
FkbO, RapK, Hyg5, and Bra8 are founder members of a previously unrecognized family of enzymes acting on chorismate, which includes the product of the bra8 gene from the pathway to the terpenoid natural product brasilicardin.
The complete genome sequence of the Streptomyces temperate phage φC31: evolutionary relationships to other viruses
The complete sequence clarifies the overall transcription map of the phage during lytic growth and the positions of elements involved in the maintenance of lysogeny and provides further evidence that dsDNA phages from diverse bacterial hosts are related and have had access to a common genetic pool.
Roles of rapH and rapG in Positive Regulation of Rapamycin Biosynthesis in Streptomyces hygroscopicus
Based on DNA sequence analysis, the products of genes rapH and rapG showed high similarities with two different families of transcriptional activators, LAL and AraC, respectively, and indicated an essential role of the RapG regulator for rapamycin biosynthesis and a supportive role of RapH.
Multiple Mutations in Hepatitis C Virus NS5A Domain II Are Required To Confer a Significant Level of Resistance to Alisporivir
It is suggested that a combination of multiple mutations in domain II of NS5A rather than a single mutation is required to render HCV significantly and universally resistant to Cyp inhibitors, in accordance with in vivo data that suggest that alisporivir is associated with a low potential for development of viral resistance.
Mutasynthesis of rapamycin analogues through the manipulation of a gene governing starter unit biosynthesis.
Separation of anti-angiogenic and cytotoxic activities of borrelidin by modification at the C17 side chain.
Rapamycin biosynthesis: Elucidation of gene product function.
- M. Gregory, Hui Hong, Rose M. Sheridan
- Chemistry, BiologyOrganic & biomolecular chemistry
- 22 September 2006
The function of gene products involved in the biosynthesis of the clinically important polyketide rapamycin were elucidated by biotransformation and gene complementation.
Synthesis, cytotoxicity, and structure–activity insight of NH- and N-methyl-3,5-bis-(arylidenyl)-4-piperidones
Twenty-one NH- and N-methyl-3,5-bis-(arylidenyl)-4-piperidone analogs of curcumin, 12 of which are novel, were synthesized and evaluated for their cytotoxicity against B16 and L1210 cells grown in culture, demonstrating that the position of the hydroxyl group is crucial for cytot toxicity.
Sangamides, a new class of cyclophilin-inhibiting host-targeted antivirals for treatment of HCV infection
Initial results of the optimisation program which led to identification of the sangamides, compounds that exhibit significantly improved potential for the treatment of chronic HCV infection are reported.