M. Grachev

Publications108
h-index22
Citations1,257
Highly Influential Citations30
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Complete sequence of the mitochondrial genome of a diatom alga Synedraacus and comparative analysis of diatom mitochondrial genomes
TLDR
This study demonstrates that pyrosequencing is an efficient method for complete sequencing of mitochondrial genomes from diatoms, and may soon give valuable information about the molecular phylogeny of this outstanding group of unicellular organisms.
Mapping of the priming substrate contacts in the active center of Escherichia coli RNA polymerase.
Studies of the functional topography of Escherichia coli RNA polymerase. A method for localization of the sites of affinity labelling.
TLDR
Evidence obtained suggests that lysine residues over the regions 1036-1066, 1234-1242, and histidine-1237 are situated in the nearest neighbourhood to, or directly involved in the formation of the active center of initiating substrate binding of the beta subunit of E. coli RNA polymerase.
The Origin and Genetic Relationships of the Baikal Seal, Phoca sibirica, by Restriction Analysis of Mitochondrial DNA
TLDR
The amount of divergence suggested that an ancestor of the Baikal seal came down to the lake approximately 0.4 million years ago as was previously indicated by paleontological studies.
Studies on the functional topography of Escherichia coli RNA polymerase. Highly selective affinity labelling by analogues of initiating substrates.
TLDR
RNA polymerase was treated in the presence of promoter-containing templates with 16 affinity reagents, derivatives on NMPs, NDPs and NTPs with reactive substituents at the terminal phosphate with super-selective affinity labelling because affinity reagent residues bound outside the active center were not elongated and thus remained non-radioactive.
Conserved motif CMLD in silicic acid transport proteins of diatoms
TLDR
Sequenced fragments of genes coding for silicon transporters (SITs) were analyzed for diatoms of evolutionarily distant classes and a hypothesis was advanced that the motif CMLD acts as a Zn-binding site, proving to be rare.
A POTENTIAL ZINC-BINDING MOTIF IN SILICIC ACID TRANSPORT PROTEINS OF DIATOMS
TLDR
In an accord with the hypothesis, Zn2+ inhibits the alkylation of cysteine and, acting as a Lewis acid, facilitates nucleophilic attack of the silicon atom by either a second molecule of silicic acid, or, more probably, a functional group of SIT.
Active center rearrangement in RNA polymerase initiation complex.
Oligonucleotides complementary to a promoter over the region -8...+2 as transcription primers for E. coli RNA polymerase.
Primer-dependent transcription by E. coli RNA polymerase on T7 promoter A2 has been studied. Synthetic deoxyribonucleotides complementary to the promoter over the region -8...+2 were taken as…
Putative silicon transport vesicles in the cytoplasm of the diatom Synedra acus during surge uptake of silicon
TLDR
Observations about the two-stage mechanism of silicon assimilation, which includes rapid silicon uptake (surge uptake) followed by slow silica deposition, are confirmed.
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