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Analysis of a piwi-Related Gene Implicates Small RNAs in Genome Rearrangement in Tetrahymena
During development of the somatic macronucleus from the germline micronucleus in ciliates, chromosome rearrangements occur in which specific regions of DNA are eliminated and flanking regions areExpand
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Macronuclear Genome Sequence of the Ciliate Tetrahymena thermophila, a Model Eukaryote
The ciliate Tetrahymena thermophila is a model organism for molecular and cellular biology. Like other ciliates, this species has separate germline and soma functions that are embodied by distinctExpand
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Phosphorylation of Histone H3 Is Required for Proper Chromosome Condensation and Segregation
Phosphorylation of histone H3 at serine 10 occurs during mitosis in diverse eukaryotes and correlates closely with mitotic and meiotic chromosome condensation. To better understand the function of H3Expand
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A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila
The Cd2+-inducible metallothionein (MTT1) gene was cloned from Tetrahymena thermophila. Northern blot analysis showed that MTT1 mRNA is not detectable in the absence of Cd2+, is induced within 10 minExpand
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Isolation of micro- and macronuclei of Tetrahymena pyriformis.
Publisher Summary This chapter discusses the isolation of micro- and macronuclei of Tetrahymena Pyriformis. With the exception of a few amicronucleate or partially amicronucleate strains ofExpand
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A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase.
Previous studies indicated that genome rearrangement involving DNA sequence elimination that occurs at late stages of conjugation in Tetrahymena is epigenetically controlled by siRNA-like scan (scn)Expand
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Histone H3 lysine 9 methylation is required for DNA elimination in developing macronuclei in Tetrahymena.
Genome-wide DNA elimination accompanies development of the somatic macronucleus from the germ-line micronucleus during the sexual process of conjugation in the ciliated protozoan TetrahymenaExpand
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Phosphorylation of histone H3 at serine 10 is correlated with chromosome condensation during mitosis and meiosis in Tetrahymena.
H3 phosphorylation has been correlated with mitosis temporally in mammalian cells and spatially in ciliated protozoa. In logarithmically growing Tetrahymena thermophila cells, for example, H3Expand
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Small RNAs in genome rearrangement in Tetrahymena.
Small RNAs produced by an RNAi-related mechanism are involved in DNA elimination during development of the somatic macronucleus from the germline micronucleus in Tetrahymena. The properties of theseExpand
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Conjugation-specific small RNAs in Tetrahymena have predicted properties of scan (scn) RNAs involved in genome rearrangement.
We proposed a scan-RNA model for genome rearrangement based on finding small RNAs that hybridized preferentially to micronuclear-specific sequences and on the properties of Twi1p, a PPD proteinExpand
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