Share This Author
Mechanism of Protein Modification by Glyoxal and Glycolaldehyde, Reactive Intermediates of the Maillard Reaction (*)
It can be estimated that approximately 50% of the CML forming in a glucose/lysine system originates from oxidation of Amadori product, and 40-50% originizes from a pre-Amadori stage largely independent from glucose autoxidation.
Mechanisms for the formation of glycoxidation products in end-stage renal disease.
The formation of the two glycoxidation products, pentosidine and CML, proceeds by different pathways and is enhanced by different precursors in the uremic milieu, evidence for the presence of increased metal-ion mediated oxidant stress in uremia.
Pathways of the Maillard reaction under physiological conditions
The present review critically discusses the relevant α-dicarbonyl compounds as central intermediates of AGE formation in vivo with a special focus on fragmentation pathways leading to formation of amide-AGEs.
Characterization of phenolic compounds in rooibos tea.
Prepative high-performance liquid chromatography was applied to obtain pure flavonoids and the purity and identity of isolated compounds was confirmed by different NMR experiments, HPLC-diode array detector (DAD), or gas chromatography-mass spectrometry (GC-MS) analysis.
Comprehensive analysis of maillard protein modifications in human lenses: effect of age and cataract.
A highly sensitive liquid chromatography-tandem mass spectrometry multimethod was developed that allowed us to quantitate 21 protein modifications in normal and cataractous lenses, respectively, and AGEs from the Amadori product and methylglyoxal were dominant.
Real-time PCR systems for the detection of the gluten-containing cereals wheat, spelt, kamut, rye, barley and oat
Homologues target sequences encoding high molecular weight (HMW) glutenin were chosen to detect wheat, kamut, spelt and rye, and the gene Hor3 was selected, showing a satisfactory ruggedness.
Protein modification by methylglyoxal: chemical nature and synthetic mechanism of a major fluorescent adduct.
- I. Shipanova, M. Glomb, R. Nagaraj
- Biology, ChemistryArchives of Biochemistry and Biophysics
- 1 August 1997
It is indicated that argpyrimidine is synthesized through an intermediate 3-hydroxypentane-2,4-dione and provide a chemical basis for fluorescence in proteins modified by methylglyoxal, suggesting that it is a major product in such modified proteins.
Isolation and characterization of glyoxal-arginine modifications.
5-(4,5-Dihydroxy-2-imino-1-imidazolidinyl)norvaline (1) was identified as the only product of the early reaction of arginine with glyoxal, which was slowly degraded to…
Medium Cut-Off (MCO) Membranes Reduce Inflammation in Chronic Dialysis Patients—A Randomized Controlled Clinical Trial
MCO-Ci Dialyzers modulate inflammation in chronic HD patients to a greater extent compared to High-flux dialyzers and Transcription of pro-inflammatory cytokines in peripheral leukocytes is markedly reduced and removal of soluble mediators is enhanced with MCO dialysis.
The Mechanism of Collagen Cross-Linking in Diabetes: A Puzzle Nearing Resolution
Experiments in the authors' laboratory revealed that ∼50% of carboxymethyllysine formed in vitro originates from pathways other than oxidation of Amadori products, i.e., most likely the oxidation of Schiff base-linked glucose.