• Publications
  • Influence
Spatial scaling of microbial eukaryote diversity
Patterns in the spatial distribution of organisms provide important information about mechanisms that regulate the diversity of life and the complexity of ecosystems. Although microorganisms mayExpand
Using the class 1 integron-integrase gene as a proxy for anthropogenic pollution
It is suggested that the relative abundance of the clinical class 1 integron-integrase gene, intI1, is a good proxy for pollution because it is linked to genes conferring resistance to antibiotics, disinfectants and heavy metals. Expand
Methods for microbial DNA extraction from soil for PCR amplification
DNA was extracted from 100g of soil using direct lysis with glass beads and SDS followed by potassium acetate precipitation, polyethylene glycol precipitation, phenol extraction and isopropanol precipitation and was compared to other DNA extraction methods with regard to DNA purity and size. Expand
Integrons: Past, Present, and Future
  • M. Gillings
  • Biology, Medicine
  • Microbiology and Molecular Reviews
  • 20 May 2014
This review examines the functions and activities of integrons before the antibiotic era and shows how antibiotic use selected particular integrons from among the environmental pool of these elements, such that integrons carrying resistance genes are now present in the majority of Gram-negative pathogens. Expand
The Evolution of Class 1 Integrons and the Rise of Antibiotic Resistance
It is shown that class 1 integrons can be found on the chromosomes of nonpathogenic soil and freshwater Betaproteobacteria, and it is concluded that environmental Betaproteinobacteria were the original source of these genetic elements. Expand
Gene flow, mobile genetic elements and the recruitment of antibiotic resistance genes into Gram-negative pathogens.
It is argued that human activities are exacerbating the problem of resistance to antibiotics by increasing the tempo of LGT and bacterial evolution for many traits that are important to humans. Expand
Gene Cassette PCR: Sequence-Independent Recovery of Entire Genes from Environmental DNA
The success of this approach indicates that mobile gene cassettes and, by inference, integrons are widespread in natural environments and are likely to contribute significantly to bacterial diversity. Expand
PCR amplification of crude microbial DNA extracted from soil
A rapid, inexpensive, large‐scale DNA extraction method involving minimal purification has been developed that is applicable to various soil types and could be used in PCR directed at high‐copy number (bacterialsmall subunit rRNA) and single‐copy genes. Expand
Continental-scale pollution of estuaries with antibiotic resistance genes
The strong correlations of identified resistance genes with known mobile elements, network analyses and partial redundancy analysis all led to the conclusion that human activity is responsible for the abundance and dissemination of these ARGs. Expand
Integrons in Xanthomonas: a source of species genome diversity.
The variety and characteristics of contemporary gene cassettes suggests that the ancestral integron had access to a diverse pool of these mobile elements, and that their genes originated outside the Xanthomonas genome. Expand