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Phase variation of a β‐1,3 galactosyltransferase involved in generation of the ganglioside GM1‐like lipo‐oligosaccharide of Campylobacter jejuni
Ganglioside mimicry by Campylobacter jejuni lipo‐oligosaccharide (LOS) is thought to be a critical factor in the triggering of the Guillain–Barré and Miller–Fisher syndrome neuropathies after C.Expand
The Genetic Bases for the Variation in the Lipo-oligosaccharide of the Mucosal Pathogen, Campylobacter jejuni
Compared the lipo-oligosaccharide biosynthesis loci from 11 Campylobacter jejunistrains expressing a total of 8 different ganglioside mimics in their LOS outer cores, at least five distinct mechanisms that allow C. jejuni to vary the structure of the LOSouter core are identified. Expand
Comparison of Campylobacter jejuni Lipooligosaccharide Biosynthesis Loci from a Variety of Sources
The hypothesis that the LOS locus is a hot spot for genetic exchange and rearrangements in C. jejuni strains is supported, with sequence analysis of two unique XL PCR products demonstrated two new LOS classes. Expand
Biosynthesis of Ganglioside Mimics in Campylobacter jejuni OH4384
We have applied two strategies for the cloning of four genes responsible for the biosynthesis of the GT1a ganglioside mimic in the lipooligosaccharide (LOS) of a bacterial pathogen,CampylobacterExpand
Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog
The first structure of a sialyltransferase is reported, CstII from Campylobacter jejuni, a highly prevalent foodborne pathogen, and structural, mutagenesis and kinetic data provide support for a novel mode of substrate binding and glycosyl transfer mechanism, including essential roles of a histidine and two tyrosine residues. Expand
Structural Characterization of Campylobacter jejuni Lipooligosaccharide Outer Cores Associated with Guillain-Barré and Miller Fisher Syndromes
It is concluded that molecular mimicry between gangliosides and C. jejuni LOS is the presumable pathogenic mechanism in most cases of C.Jejuni-related Guillain-Barré syndrome, however, the findings suggest that in some cases, other mechanisms may play a role. Expand
The crucial role of Campylobacter jejuni genes in anti-ganglioside antibody induction in Guillain-Barre syndrome.
It is demonstrated, for the first time, to the knowledge, that specific bacterial genes are crucial for the induction of anti-ganglioside antibodies. Expand
Structural and Mechanistic Analysis of Sialic Acid Synthase NeuB from Neisseria meningitidis in Complex with Mn2+, Phosphoenolpyruvate, and N-Acetylmannosaminitol*
The development of a coupled assay to monitor NeuB reaction kinetics and an 18O-labeling study that demonstrates the synthase operates via a C—O bond cleavage mechanism are reported, which suggests an essential role for the ion as an electrophilic catalyst that activates the N-acetylmannosamine carbonyl to the addition of PEP. Expand
Analysis of Campylobacter jejuni capsular loci reveals multiple mechanisms for the generation of structural diversity and the ability to form complex heptoses
For the first time, the commonality and function of a second heptose biosynthetic pathway for Campylobacter CPS independent of the pathway for lipooligosaccharide (LOS) biosynthesis is demonstrated and a novel heptosyltransferase utilized by this alternate pathway is identified. Expand
Detection of Conserved N-Linked Glycans and Phase-variable Lipooligosaccharides and Capsules from Campylobacter Cells by Mass Spectrometry and High Resolution Magic Angle Spinning NMR Spectroscopy*
The presence of this common heptasaccharide in multiple campylobacter isolates demonstrates the conservation of the N-linked protein glycosylation pathway in this organism and describes the first report of HR-MAS NMR detection of N- linked glycans on glycoproteins from intact bacterial cells. Expand