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A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage
BACKGROUND The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci. Recently it has been reported that a member of theExpand
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V(D)J recombination: RAG proteins, repair factors, and regulation.
  • M. Gellert
  • Biology, Medicine
  • Annual review of biochemistry
  • 2002
V(D)J recombination is the specialized DNA rearrangement used by cells of the immune system to assemble immunoglobulin and T-cell receptor genes from the preexisting gene segments. Because there is aExpand
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The 3' to 5' exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks.
MRE11 and RAD50 are known to be required for nonhomologous joining of DNA ends in vivo. We have investigated the enzymatic activities of the purified proteins and found that Mre11 by itself has 3' toExpand
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Nbs1 potentiates ATP-driven DNA unwinding and endonuclease cleavage by the Mre11/Rad50 complex.
The Nijmegen breakage syndrome gene product (Nbs1) was shown recently to associate in vivo with the Mre11 and Rad50 proteins, which play pivotal roles in eukaryotic DNA double-strand break repair,Expand
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Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps
Formation of double-strand breaks at recombination signal sequences is an early step in V(D)J recombination. Here we show that purified RAG1 and RAG2 proteins are sufficient to carry out thisExpand
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V(D)J recombination: a functional definition of the joining signals.
Two conserved DNA sequences serve as joining signals in the assembly of immunoglobulins and T-cell receptors from V-, (D)-, and J-coding segments during lymphoid differentiation. We have examinedExpand
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A Stable RAG1–RAG2–DNA Complex That Is Active in V(D)J Cleavage
The RAG1 and RAG2 proteins initiate V(D)J recombination by making specific double-strand DNA breaks at recombination signal sequences. We show here that RAG1 and RAG2 bind specifically to thisExpand
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DNA Transposition by the RAG1 and RAG2 Proteins A Possible Source of Oncogenic Translocations
The RAG1 and RAG2 proteins are known to initiate V(D)J recombination by making a double-strand break between the recombination signal sequence (RSS) and the neighboring coding DNA. We show that theseExpand
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Direct DNA binding by Brca1
The tumor suppressor Brca1 plays an important role in protecting mammalian cells against genomic instability, but little is known about its modes of action. In this work we demonstrate thatExpand
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The RAG1 and RAG2 Proteins Establish the 12/23 Rule in V(D)J Recombination
V(D)J recombination requires a pair of signal sequences with spacer lengths of 12 and 23 base pairs. Cleavage by the RAG1 AND RAG2 proteins was previously shown to demand only a single signalExpand
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