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Quantitative analysis of complex protein mixtures using isotope-coded affinity tags
An approach for the accurate quantification and concurrent sequence identification of the individual proteins within complex mixtures based on isotope-coded affinity tags and tandem mass spectrometry is described. Expand
Biochemistry and physiology of mammalian secreted phospholipases A2.
Phospholipases A(2) (PLA2s) are esterases that hydrolyze the sn-2 ester of glycerophospholipids and constitute one of the largest families of lipid hydrolyzing enzymes. The mammalian genome containsExpand
Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction
The design of enzymes that catalyze the bimolecular Diels-Alder reaction, a carbon-carbon bond formation reaction that is central to organic synthesis but unknown in natural metabolism, is described. Expand
5-Fluoro-2-indolyl des-chlorohalopemide (FIPI), a Phospholipase D Pharmacological Inhibitor That Alters Cell Spreading and Inhibits Chemotaxis
5-fluoro-2-indolyl des-chlorohalopemide (FIPI) is examined, and it is shown that it rapidly blocks in vivo PA production with subnanomolar potency, indicating potential utility for it as a therapeutic for autoimmunity and cancer metastasis. Expand
Slow- and tight-binding inhibitors of the 85-kDa human phospholipase A2.
A trifluoromethyl ketone analogue of arachidonic acid in which the COOH group is replaced with COCF3 (AACOCF3) was prepared and found to be a tight- and slow-binding inhibitor of the 85-kDa cytosolicExpand
Serine 727 Phosphorylation and Activation of Cytosolic Phospholipase A2 by MNK1-related Protein Kinases*
It is shown that phosphorylation of cPLA2 at both Ser-505 and Ser-727 and elevation of Ca2+ leads to its activation in agonist-stimulated cells and suggests that MNK1 or a closely related kinase is responsible for in vivo phosphorylated of c PLA2 onSer-727. Expand
Blocking protein farnesyltransferase improves nuclear blebbing in mouse fibroblasts with a targeted Hutchinson-Gilford progeria syndrome mutation.
A gene-targeted mouse model of HGPS was created, genetically identical primary mouse embryonic fibroblasts were generated, and the effect of a farnesyltransferase inhibitor on nuclear blebbing was examined, suggesting a possible treatment strategy for HGPS. Expand
Inhibition of lipoprotein-associated phospholipase A2 reduces complex coronary atherosclerotic plaque development
The data show that selective inhibition of Lp-PLA2 inhibits progression to advanced coronary atherosclerotic lesions and confirms a crucial role of vascular inflammation independent from hypercholesterolemia in the development of lesions implicated in the pathogenesis of myocardial infarction and stroke. Expand
Direct multiplex assay of lysosomal enzymes in dried blood spots for newborn screening.
A multiplex screening method for all five lysosomal enzymes that uses newborn-screening cards containing dried blood spots as the enzyme source that can be readily automated, and the anticipated reagent and supply costs are well within the budget limits of newborn- Screening centers. Expand
Interfacial Kinetic and Binding Properties of the Complete Set of Human and Mouse Groups I, II, V, X, and XII Secreted Phospholipases A2 *
A dramatic correlation exists between the ability of the sPLA2s to hydrolyze phosphatidylcholine-rich vesicles efficiently in vitro and the ability to release arachidonic acid when added exogenously to mammalian cells; the group V and X s PLA2s are uniquely efficient in this regard. Expand