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Quantitative analysis of complex protein mixtures using isotope-coded affinity tags
- S. Gygi, B. Rist, S. Gerber, F. Tureček, M. Gelb, R. Aebersold
- BiologyNature Biotechnology
- 1 October 1999
An approach for the accurate quantification and concurrent sequence identification of the individual proteins within complex mixtures based on isotope-coded affinity tags and tandem mass spectrometry is described.
Biochemistry and physiology of mammalian secreted phospholipases A2.
Phospholipases A(2) (PLA2s) are esterases that hydrolyze the sn-2 ester of glycerophospholipids and constitute one of the largest families of lipid hydrolyzing enzymes. The mammalian genome contains…
Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction
The design of enzymes that catalyze the bimolecular Diels-Alder reaction, a carbon-carbon bond formation reaction that is central to organic synthesis but unknown in natural metabolism, is described.
Slow- and tight-binding inhibitors of the 85-kDa human phospholipase A2.
A trifluoromethyl ketone analogue of arachidonic acid in which the COOH group is replaced with COCF3 (AACOCF3) was prepared and found to be a tight- and slow-binding inhibitor of the 85-kDa cytosolic…
Blocking protein farnesyltransferase improves nuclear blebbing in mouse fibroblasts with a targeted Hutchinson-Gilford progeria syndrome mutation.
A gene-targeted mouse model of HGPS was created, genetically identical primary mouse embryonic fibroblasts were generated, and the effect of a farnesyltransferase inhibitor on nuclear blebbing was examined, suggesting a possible treatment strategy for HGPS.
Direct multiplex assay of lysosomal enzymes in dried blood spots for newborn screening.
A multiplex screening method for all five lysosomal enzymes that uses newborn-screening cards containing dried blood spots as the enzyme source that can be readily automated, and the anticipated reagent and supply costs are well within the budget limits of newborn- Screening centers.
5-Fluoro-2-indolyl des-chlorohalopemide (FIPI), a Phospholipase D Pharmacological Inhibitor That Alters Cell Spreading and Inhibits Chemotaxis
5-fluoro-2-indolyl des-chlorohalopemide (FIPI) is examined, and it is shown that it rapidly blocks in vivo PA production with subnanomolar potency, indicating potential utility for it as a therapeutic for autoimmunity and cancer metastasis.
Platelets release mitochondria serving as substrate for bactericidal group IIA-secreted phospholipase A2 to promote inflammation.
It is shown that activated platelets release respiratory-competent mitochondria, both within membrane-encapsulated microparticles and as free organelles, at the midpoint of a potent mechanism leading to inflammatory responses.
Serine 727 Phosphorylation and Activation of Cytosolic Phospholipase A2 by MNK1-related Protein Kinases*
- Y. Hefner, A. Börsch-Haubold, M. Gelb
- Biology, ChemistryJournal of Biological Chemistry
- 1 December 2000
It is shown that phosphorylation of cPLA2 at both Ser-505 and Ser-727 and elevation of Ca2+ leads to its activation in agonist-stimulated cells and suggests that MNK1 or a closely related kinase is responsible for in vivo phosphorylated of c PLA2 onSer-727.
Interfacial Kinetic and Binding Properties of the Complete Set of Human and Mouse Groups I, II, V, X, and XII Secreted Phospholipases A2 *
A dramatic correlation exists between the ability of the sPLA2s to hydrolyze phosphatidylcholine-rich vesicles efficiently in vitro and the ability to release arachidonic acid when added exogenously to mammalian cells; the group V and X s PLA2s are uniquely efficient in this regard.