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Isolation and biochemical characterization of a thaumatin-like kiwi allergen.
This study reported isolation and full characterization of a new kiwi allergen, TLP (isoelectric points of 9.4 and 9.5 and molecular weight of 24 kd), which belongs to the family of pathogenesis-related proteins. Expand
A novel recombinantly produced banana lectin isoform is a valuable tool for glycoproteomics and a potent modulator of the proliferation response in CD3+, CD4+, and CD8+ populations of human PBMCs.
This recombinant BanLec is a useful reagent for glycoproteomics and lectin microarrays, with a potential for modulation of the immune response. Expand
Immediate allergic reactions to cephalosporins and penicillins and their cross‐reactivity in children
The frequency of immediate allergic reactions to cephalosporins is considerably lower compared to penicillins, and the degree of cross‐reactivity depends on the generation of cep HalOSporins, being higher with earlier generation cepHalosporin and higher with later generation penicllins. Expand
Cloning, Heterologous Expression, Purification and Characterization of M12 Mutant of Aspergillus niger Glucose Oxidase in Yeast Pichia pastoris KM71H
M12 mutant of GOx produced in P. pastoris KM71H could be useful for manufacturing of glucose biosensors and biofuel cells and showed three times higher activity compared to the wt GOx toward redox mediators like N,N-dimethyl-nitroso-aniline used for glucose strips manufacturing. Expand
Yeast surface display for the expression, purification and characterization of wild-type and B11 mutant glucose oxidases.
The kinetic properties of the fusion proteins displayed the same ratio as their native counterparts, confirming that yeast surface display is suitable for the high-throughput directed evolution of GOx using flow cytometry for selection. Expand
Allergenic potency of kiwi fruit during fruit development
Two major allergens, Act c 1 and Act c 2, mainly accounted for the highest allergenic potential of November kiwi extract in vivo and in vitro and should be taken into account during preparation of extracts for allergy diagnosis. Expand
The identification of a low molecular mass bacteriocin, rhamnosin A, produced by Lactobacillus rhamnosus strain 68
Aims:  This study focuses on the isolation and characterization of a peptide with bacteriocin‐like properties isolated from Lactobacillus rhamnosus strain 68, previously identified by 16S rRNA geneExpand
In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit.
Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules. Expand
Evaluation of the thermal stability and digestibility of heterologously produced banana lectin.
Abstract The thermal stability of recombinant mannose-specific banana lectin (rBanLec), as well as its stability under conditions of simulated gastro-intestinal fluid (SGF), was investigated. rBanLecExpand