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ITS primers with enhanced specificity for basidiomycetes ‐ application to the identification of mycorrhizae and rusts
ITS1‐F/ITS4‐B preferential amplification was shown to be particularly useful for detection and analysis of the basidiomycete component in ectomycorrhizae and in rust‐infected tissues. Expand
Community structure of ectomycorrhizal fungi in a Pinus muricata forest: above- and below-ground views
By using molecular methods based on polymerase chain reaction, this work was able to identify the fungal symbionts directly from mycorrhizae in nearly all of the myCorrhizal morphotypes the authors found. Expand
Mycorrhizal Symbioses and Plant Invasions
A conceptual framework for considering mycorrhizal symbioses in plant species invasions is developed and it is shown that aspects of this symbiosis can critically influence the trajectory of a plant invasion. Expand
Identification of indigenous and introduced symbiotic fungi in ectomycorrhizae by amplification of nuclear and mitochondrial ribosomal DNA
The polymerase chain reaction was used to amplify specific regions of the nuclear and mitochondrial genomes of fungi using DNA extracted from pure cultures as well as that directly from ectomycorrhizal rootlets, providing a sensitivity and specificity sufficient for detection and analysis of a single mycorrhIZal rootlet. Expand
Diversity and specificity of ectomycorrhizal fungi retrieved from an old-growth Mediterranean forest dominated by Quercus ilex.
The ectomycorrhizal fungal diversity in a Mediterranean old-growth Quercus ilex forest stand from Corsica (France), where Arbutus unedo was the only other ECM host, was analysed to determine whether oak age and host species shaped below-ground ECM diversity. Expand
A sequence database for the identification of ectomycorrhizal basidiomycetes by phylogenetic analysis
A sequence database for 80 genera of Basidiomycota from the Hymenomycete lineage for a small region of the mitochondrial large subunit rRNA gene is assembled, and two interesting patterns emerge: the switch between ectomycorrhizae and saprobic lifestyles appears to have happened convergently several and perhaps many times. Expand
Genetic structure of a natural population of the ectomycorrhizal fungus Suillus pungens
The combination of extensive vegetative growth and abundant fruiting suggests S. pungens utilizes more carbon than might be expected for a species which accounts for <3% of the total ectomycorrhizal abundance at the site, and genetic analysis shows that although out-crossing appears to predominate in the population, at least a low percentage of spores are secondarily homothallic. Expand
ITS-RFLP matching for identification of fungi.
Mycorrhizal diversity in arctic and alpine tundra: an open question
Molecular methods to identify mycorrhizal fungi on plant roots will resolve questions related to the structure and dynamics of communities of mycorinthian fungi in arctic and alpine tundra. Expand
Distribution and drivers of ectomycorrhizal fungal communities across the North American Arctic
This first large-scale study of EMF communities across all five bioclimatic subzones of the North American Arctic, accompanied by an extensive set of environmental factors analyzed to date, suggests that with ongoing climate warming, EMF community composition may be affected by northward shifts of some taxa. Expand