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Hydrolysis of bis(5'-nucleosidyl) polyphosphates by Escherichia coli 5'-nucleotidase.
A different enzyme activity that hydrolyzes dinucleoside polyphosphates is described, which was purified from sonic extracts and osmotic shock fluid and was able to split diadenosine triphosphate and adenosine-(5')-diphospho-(5)-beta-D-ribose, ribose-5-ph phosphate, and dinucleose polyph phosphates. Expand
T4 DNA ligase synthesizes dinucleoside polyphosphates
The present report may be related to the recent finding that human Fhit (fragile histidine triad) protein, encoded by the FHIT putative tumor suppressor gene, is a typical dinucleoside 5′,5″‐P1,P3‐triphosphate (Ap3A) hydrolase (EC Expand
In Saccharomyces cerevisiae, the effect of H2O2 on ATP, but not on glyceraldehyde-3-phosphate dehydrogenase, depends on the glucose concentration
As has been previously shown, Saccharomyces cerevisiae grown in 2% or 0.025% glucose uses this carbohydrate by the fermentative or oxidative pathways, respectively, and the effect of the addition of H2O2 on the level of ATP and GAPDH activity differed. Expand
T4 RNA ligase catalyzes the synthesis of dinucleoside polyphosphates.
The specificity of a compound to be acceptor of AMP from the E-AMP complex was very broad, and with Km values between 1 and 2 mm, and the relative rate of synthesis of the following radioactive (di)nucleotides was observed. Expand
H2O2, but not menadione, provokes a decrease in the ATP and an increase in the inosine levels in Saccharomyces cerevisiae. An experimental and theoretical approach.
The strong decrease in the ATP level upon H2O2 treatment is an important factor to be considered for understanding the response of yeast, and probably other cell types, to oxidative stress. Expand
Metabolic fate of AMP, IMP, GMP and XMP in the cytosol of rat brain: an experimental and theoretical analysis
A systematic study of the metabolic fate of AMP, IMP, GMP and XMP (NMP) in the presence of cytosol from rat brain is here presented; the kinetics of both disappearance of NMP, and appearance of theirExpand
Uridine 5′‐polyphosphates (p4U and p5U) and uridine(5′)polyphospho(5′)nucleosides (Up n Ns) can be synthesized by UTP:glucose‐1‐phosphate uridylyltransferase from Saccharomyces cerevisiae
This is the first example of an enzyme that may be responsible for accumulation of dinucleoside tetraphosphates containing two pyrimidine nucleosides in vivo. Expand
Time course of luciferyl adenylate synthesis in the firefly luciferase reaction
The time course of luciferyl adenylate formation in the reaction catalyzed by firefly luciferase (EC has been followed and the potential use of luc iferyl adnylate for luciferases detection is proposed. Expand
Thermostable Pyrococcus furiosus DNA ligase catalyzes the synthesis of (di)nucleoside polyphosphates
Pfu DNA ligase is used in the ligase chain reaction (LCR) and some of the reactions here reported [in particular the synthesis of Ap4adenosine (Ap4A)] could take place during the course of that reaction. Expand
Two low Km hydrolytic activities on dinucleoside 5',5"'-P1,P4-tetraphosphates in rat liver. Characterization as the specific dinucleoside tetraphosphatase and a phosphodiesterase I-like enzyme.
Particulate activity, as extracted with Triton X-100, is composed of two enzymes separable by gel filtration, and the possible identity of this activity with phosphodiesterase I is discussed, and its potential role in the metabolism of dinucleoside tetraphosphates is indicated. Expand