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Molecular characterization and cell-specific expression of an ion transport peptide in the tobacco hornworm, Manduca sexta
TLDR
A nucleotide fragment spanning the conserved A1/A2 region of the putative CHH/ITP gene was sequenced from larval cDNA of the tobacco hornworm, Manduca sexta and showed a high degree of sequence conservation with the same region from other insects and, to a lesser degree, with that of crustacean species, suggesting the presence of a Manduca-specific CHH /ITP mRNA.
Classroom sound can be used to classify teaching practices in college science courses
TLDR
The development and application of the machine-learning–derived algorithm Decibel Analysis for Research in Teaching (DART), which can analyze thousands of hours of STEM course audio recordings quickly, with minimal costs, and without need for human observers is described.
Investigating Instructor Talk in Novel Contexts: Widespread Use, Unexpected Categories, and an Emergent Sampling Strategy
TLDR
The vast majority of Instructor Talk could be characterized using the originally published Instructor Talk framework, suggesting the robustness of this framework and a new form of Instructor talk—Negatively Phrased Instructor Talk, language that may discourage students or distract from the learning process—was detected in these novel course contexts.
The distribution and partial characterization of FMRFamide-related peptides in the salivary glands of the locust, Locusta migratoria
TLDR
High performance liquid chromatography of salivary gland tissue extracts revealed 4 peaks of immunoreactive material, 2 of which co-migrated with AFIRFamide and GQERNFLRFamide, previously isolated from the locust ventral nerve cord, which did not elevate basal levels of the second messengers cyclic AMP or cyclic GMP in thesalivary glands.
Central neural alterations predominate in an insect model of nociceptive sensitization
TLDR
Novel in situ and in vitro preparations based on paired extracellular nerve recordings and videography identify central and peripheral nerves responsible for nociception and sensitization of the defensive behavior in Manduca sexta and suggest that M. sextA may be a valuable model for studying the electrophysiological properties of nocICEptive sensitization and potentially related conditions such as allodynia and hyperalgesia.
In situ hybridization analysis of leucomyosuppressin mRNA expression in the cockroach, Diploptera punctata
TLDR
Immunohistochemistry with a polyclonal antiserum generated against FMRFamide, but which recognizes extended RFamide peptides, demonstrated numerous RFamide‐like immunoreactive cells and processes in both nervous and nonnervous tissues, suggesting that LMS may play a central role in Diploptera and may be associated with feeding and digestion.
Modulation of ecdysis in the moth Manduca sexta: the roles of the suboesophageal and thoracic ganglia.
TLDR
CO(2) therefore appears to be a novel and non-invasive tool for studies of ecdysis behavior in insects and can uncouple the basic larval motor program from modulatory influences provided by the adult pterothoracic ganglion.
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