• Publications
  • Influence
Biocontrol of Ralstonia solanacearum by Treatment with Lytic Bacteriophages
An alternative biocontrol method using a unique phage, such as φRSL1, instead of a phage cocktail with highly virulent phages is proposed, which resulted in effective prevention of wilting in R. solanacearum. Expand
New bacteriophages that infect the phytopathogen Ralstonia solanacearum.
Four kinds of bacteriophage were isolated from Ralstonia solanacearum, a soil-borne Gram-negative bacterium that is the causative agent of bacterial wilt in many important crops, and characterized as Ff-type phages (Inovirus) based on their particle morphology, genomic ssDNA and infection cycle. Expand
Aminoacylation of tRNAs encoded by Chlorella virus CVK2.
Results provide evidence that some components of the host protein synthesis machinery can be replaced by viral gene products, and are the first report of tRNA aminoacylation encoded by viruses of eukaryotes. Expand
Behavior of organelles and their nucleoids in the shoot apical meristem during leaf development in Arabidopsis thaliana L.
The behavior of organelle nucleoids and cell nuclei was studied in the shoot apical meristem and developing first foliage leaves of Arabidopsis thaliana and DNA synthesis in situ strongly suggests that the amount of mitochondrial DNA per mitochondrion is gradually reduced due to continual divisions of the mitochondria during low levels of mitochondrialDNA synthesis. Expand
Zepp, a LINE‐like retrotransposon accumulated in the Chlorella telomeric region
Detailed sequence analysis of the Chlorella subtelomeric region revealed a novel mechanism of Zepp transposition; successive insertions of each Zepp element into another Zepp as a target, leaving a tandem array of their 3′‐regions with poly(A) tracts facing toward the centromere. Expand
Loss of virulence of the phytopathogen Ralstonia solanacearum through infection by φRSM filamentous phages.
Changes related to the virulence in φRSM3-infected cells, including reduced twitching motility and reduced amounts of type IV pili (Tfp), lower levels of β-1,4-endoglucanase (Egl) activity and extracellular polysaccharides (EPS) production, and reduced expression of certain genes are characterized. Expand
Immediate early genes expressed in chlorovirus infections.
Twenty-three chlorovirus genes expressed in host cells as early as 5-10 min postinfection (p.i.), or immediate early, were isolated and characterized. Some showed significant homology with those forExpand
The filamentous phage ϕRSS1 enhances virulence of phytopathogenic Ralstonia solanacearum on tomato.
It is shown that R. solanacearum infected by ϕRSS1 becomes more virulent on host plants, and induced early expression of phcA, the global virulence regulator, resulting in early wilting. Expand
Genetic variation of chlorella viruses: variable regions localized on the CVK2 genomic DNA.
The gene arrangement along the genome was compared between CVK2 and PBCV-1, the prototypic member of Phycodnaviridae, whose genomic sequence is now available and extensive colinearity was revealed. Expand
Fluorescence microscopic study of the formation of giant mitochondrial nuclei in the young ovules ofPelargonium zonale
The giant mitochondrial nuclei which have the capacity for including the master mitochondrial genome in the young ovaries of Pelargonium zonale are found by use of a 4′-6-diamidino-2-phenylindole (DAPI) epifluorescence microscopy, a Technovit embedding, and a video-intensified photon counting system. Expand