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Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
The efficacy of this cDNA cloning strategy was demonstrated by isolating cDNA clones of mRNA from int-2, a mouse gene that expresses four different transcripts at low abundance, the longest of which is approximately 2.9 kilobases. Expand
REST: A mammalian silencer protein that restricts sodium channel gene expression to neurons
It is proposed that expression of the type II sodium channel gene in neurons reflects a default pathway that is blocked in nonneuronal cells by the presence of REST. Expand
Spatially restricted expression of Dlx-1, Dlx-2 (Tes-1), Gbx-2, and Wnt- 3 in the embryonic day 12.5 mouse forebrain defines potential transverse and longitudinal segmental boundaries
A model for forebrain segmentation is suggested based on patterns of expression found within the dorsal thalamus for the Gbx- 2 and Wnt-3 genes, consistent with neuromeric theories of forebrain development. Expand
Phosphatidylinositol 4-Phosphate 5-Kinase α Is a Downstream Effector of the Small G Protein ARF6 in Membrane Ruffle Formation
PI(4)P5Kalpha is a downstream effector of ARF 6 and when ARF6 is activated by agonist stimulation, it triggers recruitment of a diverse but interactive set of signaling molecules into sites of active cytoskeletal and membrane rearrangement. Expand
Rapid amplification of complementary DNA ends for generation of full-length complementary DNAs: thermal RACE.
  • M. Frohman
  • Biology, Medicine
  • Methods in enzymology
  • 1993
Thermal rapid amplification of cDNA ends (RACE) is a polymerase chain reaction (PCR) technique through which previously unobtained 3' and 5' ends of a cDNA can be amplified starting with the knowledge of a small stretch of sequence from within an internal region of the cDNA. Expand
Phospholipase D2, a distinct phospholipase D isoform with novel regulatory properties that provokes cytoskeletal reorganization
PLD2 is a newly identified mammalian PLD isoform with novel regulatory properties that provokes cortical reorganization and undergoes redistribution in serum-stimulated cells, suggesting that it may have a role in signal-induced cytoskeletal regulation and/or endocytosis. Expand
Characterization of Two Alternately Spliced Forms of Phospholipase D1
  • S. Hammond, J. Jenco, +7 authors Andrew J. Morris
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • 7 February 1997
An evolutionarily conserved shorter splice variant of PLD1 lacking 38 amino acids is identified that arises from regulated splicing of an alternate exon, suggesting that these three classes of regulators interact with different sites on the enzyme. Expand
Mutagenesis of phospholipase D defines a superfamily including a trans‐Golgi viral protein required for poxvirus pathogenicity
The results suggest that vaccinia virus and hPLD1 may act through analogous mechanisms to effect viral cellular egress and vesicular trafficking, respectively. Expand
Human ADP-ribosylation Factor-activated Phosphatidylcholine-specific Phospholipase D Defines a New and Highly Conserved Gene Family (*)
The identification of the first human PLD cDNA is reported, which defines a new and highly conserved gene family and likely encodes the gene product responsible for the most widely studied endogenous PLD activity. Expand
Phospholipase D: a lipid centric review
This review focuses on the lipid precursors and products of mammalian PLD metabolism, especially phosphatidic acid and the roles this lipid performs in the mediation of the functions of PLD. Expand