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The two nonstructural proteins from wheat dwarf virus involved in viral gene expression and replication are retinoblastoma-binding proteins.
TLDR
It is demonstrated for the first time that the C1:C2 protein from WDV interacts with a retinoblastoma protein, providing an explanation for the previously observed dependence of viral replication on an intact Rb-binding motif.
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Production of Galacto-oligosaccharides by the β-Galactosidase from Kluyveromyces lactis : comparative analysis of permeabilized cells versus soluble enzyme.
TLDR
Results were correlated with the higher stability of β-galactosidase when permeabilized whole cells were used, and the typical profile with a maximum GOS concentration followed by partial hydrolysis of the newly formed oligosaccharides was not observed with the soluble enzymes.
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Characterization of a beta-fructofuranosidase from Schwanniomyces occidentalis with transfructosylating activity yielding the prebiotic 6-kestose.
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Molecular and Biochemical Characterization of a β-Fructofuranosidase from Xanthophyllomyces dendrorhous
TLDR
It is demonstrated that the Xd-INV could functionally complement the suc2 mutation of Saccharomyces cerevisiae and a structural model of the new enzyme based on the homologous invertase from Arabidopsis thaliana has also been obtained.
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New Insights into the Fructosyltransferase Activity of Schwanniomyces occidentalis β-Fructofuranosidase, Emerging from Nonconventional Codon Usage and Directed Mutation
TLDR
Modeling the transfructosylation products into the active site, in combination with an analysis of the kinetics and transfructOSylation reactions, defined a new region responsible for the transferase specificity of the enzyme.
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"Dried alginate-entrapped enzymes (DALGEEs) and their application to the production of fructooligosaccharides"
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Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity
TLDR
Production and understanding of how the enzymes generating bioactive chito-oligomers work is essential for their biotechnological application, and paves the way for future work to take advantage of chitinolytic activities.
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Structural and Kinetic Insights Reveal That the Amino Acid Pair Gln-228/Asn-254 Modulates the Transfructosylating Specificity of Schwanniomyces occidentalis β-Fructofuranosidase, an Enzyme That…
TLDR
The crystal structure of inactivated Ffase complexed with fructosylnystose and inulin shows the intricate net of interactions keeping the substrate tightly bound at the active site, giving the first experimental evidence of the direct role of a GH32 β-sandwich domain in substrate binding.
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Tailored Enzymatic Synthesis of Chitooligosaccharides with Different Deacetylation Degrees and Their Anti-Inflammatory Activity
By controlled hydrolysis of chitosan or chitin with different enzymes, three types of chitooligosaccharides (COS) with MW between 0.2 and 1.2 kDa were obtained: fully deacetylated (fdCOS), partially…
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Assessment of Schwanniomyces occidentalis as a host for protein production using the wide-range Xplor®2 expression platform
TLDR
The extension of the use of the wide-range expression platform Xplor®2 to another yeast species of biotechnological interest is demonstrated and affects auxotrophy recovery as well as protein expression.
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