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Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins.
- J. Llopis, J. McCaffery, A. Miyawaki, M. Farquhar, R. Tsien
- BiologyProceedings of the National Academy of Sciences…
- 9 June 1998
The pH of the medial/trans-Golgi was measured and demonstrated that the Golgi membrane in intact cells is relatively permeable to H+, and that Cl- serves as a counter-ion for H+ transport and likely helps to maintain electroneutrality.
JUNCTIONAL COMPLEXES IN VARIOUS EPITHELIA
The tight junction is impervious to concentrated protein solutions and appears to function as a diffusion barrier or "seal," and the desmosome and probably also the zonula adhaerens may represent intercellular attachment devices.
GOLPH3 Bridges Phosphatidylinositol-4- Phosphate and Actomyosin to Stretch and Shape the Golgi to Promote Budding
THE DEVELOPMENT OF NEUTROPHILIC POLYMORPHONUCLEAR LEUKOCYTES IN HUMAN BONE MARROW
The findings indicate that the situation is basically the same as described previously in the rabbit, insofar as the origin, enzymic activity, and persistence in the mature cell of the two types of granules are concerned.
CELL JUNCTIONS IN AMPHIBIAN SKIN
Interpreted in the light of current physiological data, these findings suggest that the diffusion of water, ions, and small, water-soluble molecules is impeded along the intercellular spaces of the epidermis by zonulae occludentes while it is facilitated from cell to cell within the epidesis by z onulae and maculae OccludENTes.
The tight junction protein ZO-1 is concentrated along slit diaphragms of the glomerular epithelium
The distribution of ZO-1 and the close developmental relationship between the two junctions suggest that the slit diaphragm is a variant of the tight junction that shares with it at least one structural protein and the functional property of defining distinctive plasmalemmal domains.
Podocalyxin activates RhoA and induces actin reorganization through NHERF1 and Ezrin in MDCK cells.
- S. Schmieder, Masaaki Nagai, R. Orlando, T. Takeda, M. Farquhar
- BiologyJournal of the American Society of Nephrology
- 1 September 2004
It was found that full-length PC but not the PC mutant is connected to actin, induces redistribution of actin toward the apical membrane, and leads to increased RhoA activity.
The Golgi apparatus (complex)-(1954-1981)-from artifact to center stage
The regulator of G protein signaling family.
- L. de Vries, B. Zheng, T. Fischer, E. Elenko, M. Farquhar
- Biology, ChemistryAnnual Review of Pharmacology and Toxicology
What has been learned so far about the role of RGS proteins in regulating G protein-coupled receptor signaling is discussed and areas that may be fruitful for future research are pointed out.
Increased permeability of the glomerular basement membrane to ferritin after removal of glycosaminoglycans (heparan sulfate) by enzyme digestion
It is demonstrated that removal of heparan sulfate (but not other GAG) leads to a dramatic increase in the permeability of the GBM to NF.