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Expression cloning of a cDNA encoding UDP-GlcNAc:Gal beta 1-3-GalNAc-R (GlcNAc to GalNAc) beta 1-6GlcNAc transferase by gene transfer into CHO cells expressing polyoma large tumor antigen.
- M. F. Bierhuizen, M. Fukuda
- Biology, Medicine
- Proceedings of the National Academy of Sciences…
- 1 October 1992
A cDNA encoding UDP-GlcNAc:Gal beta 1-3GalNAc-R (GlcNAc to GalNAc) beta 1-6GlcNAc transferase (EC 18.104.22.168), which forms critical branches in O-glycans, has been isolated by an expression cloning… Expand
Expression of the developmental I antigen by a cloned human cDNA encoding a member of a beta-1,6-N-acetylglucosaminyltransferase gene family.
The blood group i/I antigens were the first identified alloantigens that display a dramatic change during human development. The i and I antigens are determined by linear and branched… Expand
Expression of a differentiation antigen and poly-N-acetyllactosaminyl O-glycans directed by a cloned core 2 beta-1,6-N-acetylglucosaminyltransferase.
- M. F. Bierhuizen, K. Maemura, M. Fukuda
- Biology, Medicine
- The Journal of biological chemistry
- 11 February 1994
Chinese hamster ovary (CHO) cells do not contain detectable amounts of core 2 beta-1,6-N-acetylglucosaminyltransferase, C2GnT, and thus lack various modifications in their branched O-linked… Expand
Glycosylation of three molecular forms of human α1‐acid glycoprotein having different interactions with concanavalin A
Human αl-acid glycoprotein (AGP) was separated into a non-bound (AGP-A; 46%), a retarded (AGP-B; 39%) and a bound fraction (AGP-C; 150%) using concanavalin A (ConA)-Sepharose chromatography. The… Expand
Removal of the N-terminal part of alfalfa mosaic virus coat protein interferes with the specific binding to RNA 1 and genome activation.
Trypsinized coat protein of alfalfa mosaic virus lacking 25 amino acids at its N terminus still has the capability to form complexes with RNA which are detectable by sedimentation in sucrose… Expand
Expression cloning of glycosyltransferases.
Cloning of cDNAs encoding glycosyltransferases enters into a new era because of the advent of expression cloning for glycosyltransferases. This mini-review summarizes a short historical view on the… Expand
Coat protein binding sites on RNA 1 of alfalfa mosaic virus.
- D. Zuidema, M. F. Bierhuizen, B. Cornelissen, J. Bol, E. Jaspars
- Biology, Medicine
- 1 March 1983
The largest genome segment, RNA 1, of alfalfa mosaic virus forms complexes with viral coat protein. These complexes were subjected to digestion with ribonucleases T1 or A and filtered onto Millipore… Expand
Genomic organization of core 2 and I branching beta-1,6-N-acetylglucosaminyltransferases. Implication for evolution of the beta-1,6-N-acetylglucosaminyltransferase gene family.
Two human beta-1,6-N-acetylglucosaminyltransferases forming the core 2 O-glycan branch, C2GnT and the I antigen, IGnT, are homologous to each other in three regions of the catalytic domain (A, B, C)… Expand
Effect of α(2–6)-linked sialic acid and α(1–3)-linked fucose on the interaction ofN-linked glycopeptides and related oligosaccharides with immobilizedPhaseolus vulgaris leukoagglutinating lectin…
- M. F. Bierhuizen, Hommo Tedzes, W. E. Schiphorst, D. H. V. D. Eijnden, W. Dijk
- Glycoconjugate Journal
- 1 March 1988
The effects of branching and substitution of branches by sialic acid and fucose on the interaction ofN-linked glycopeptides and related oligosaccharides with immobilizedPhaseolus vulgaris… Expand
Isolation and characterization of a pseudogene related to human core 2 β-1,6-N-acetylglucosaminyltransferase
In a previous study, we isolated genomic clones encoding core 2β-1,6-N-acetylglucosaminyltransferase (C2GnT) and blood group IGnT and proposed that these two genes were produced from a common… Expand