• Publications
  • Influence
Stress resistance in Saccharomyces cerevisiae is strongly correlated with assembly of a novel type of multiubiquitin chain.
The covalent attachment of ubiquitin (Ub) to short-lived or damaged proteins is believed to be the signal that initiates their selective degradation. In several cases, it has been shown that theExpand
  • 257
  • 14
Distinct regulation of Ubc13 functions by the two ubiquitin-conjugating enzyme variants Mms2 and Uev1A
Ubc13, a ubiquitin-conjugating enzyme (Ubc), requires the presence of a Ubc variant (Uev) for polyubiquitination. Uevs, although resembling Ubc in sequence and structure, lack the active siteExpand
  • 150
  • 14
  • PDF
Epitope-tagged ubiquitin. A new probe for analyzing ubiquitin function.
In the present work, a method based on an epitope-tagged ubiquitin derivative is described that allows for the unambiguous detection of ubiquitin-protein conjugates formed in vivo or in vitro.Expand
  • 202
  • 11
Noncovalent Interaction between Ubiquitin and the Human DNA Repair Protein Mms2 Is Required for Ubc13-mediated Polyubiquitination*
Ubiquitin-conjugating enzyme variants share significant sequence similarity with typical E2 (ubiquitin-conjugating) enzymes of the protein ubiquitination pathway but lack their characteristic activeExpand
  • 116
  • 9
  • PDF
A Single Mms2 “Key” Residue Insertion into a Ubc13 Pocket Determines the Interface Specificity of a Human Lys63 Ubiquitin Conjugation Complex*
Human Ubc13 and Mms2 (or its homolog, Uev1) form a unique ubiquitin-conjugating enzyme (Ubc) complex that generates atypical Lys63-linked ubiquitin conjugates. Such conjugates are attached toExpand
  • 47
  • 9
The short-lived MAT alpha 2 transcriptional regulator is ubiquitinated in vivo.
The substrates of ubiquitin-dependent proteolytic pathways include both damaged or otherwise abnormal proteins and undamaged proteins that are naturally short-lived. Few specific examples of theExpand
  • 197
  • 6
Coarse-grained molecular simulation of diffusion and reaction kinetics in a crowded virtual cytoplasm.
We present a general-purpose model for biomolecular simulations at the molecular level that incorporates stochasticity, spatial dependence, and volume exclusion, using diffusing and reactingExpand
  • 165
  • 5
  • PDF
Identification of a Novel Membrane Transporter Associated with Intracellular Membranes by Phenotypic Complementation in the Yeast Saccharomyces cerevisiae(*)
A partial mouse cDNA was isolated by its ability to functionally complement a thymidine transport deficiency in plasma membranes of the yeast, Saccharomyces cerevisiae. The full-length cDNA encoded aExpand
  • 54
  • 5
Crystal structure of the human ubiquitin conjugating enzyme complex, hMms2–hUbc13
The ubiquitin conjugating enzyme complex Mms2–Ubc13 plays a key role in post-replicative DNA repair in yeast and the NF-κB signal transduction pathway in humans. This complex assembles novelExpand
  • 147
  • 4
  • PDF
Cdc34 Self-Association Is Facilitated by Ubiquitin Thiolester Formation and Is Required for Its Catalytic Activity
ABSTRACT Using a coimmunoprecipitation strategy, we showed that the Cdc34 ubiquitin (Ub)-conjugating enzyme from Saccharomyces cerevisiae self-associates in cell lysates, thereby indicating an inExpand
  • 52
  • 4