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Succinate production in dual-phase Escherichia coli fermentations depends on the time of transition from aerobic to anaerobic conditions
Six transition times based on physiological states were selected for studying dual-phase fermentations of Escherichia coli AFP111 and the final succinic acid yield and productivity depend greatly on the physiological state of the cells at the time of transition.
Effects of Growth Mode and Pyruvate Carboxylase on Succinic Acid Production by Metabolically Engineered Strains of Escherichia coli
Enzyme assays indicated that under dual-phase conditions, carbon flows not only through the reductive arm of the tricarboxylic acid cycle for succinate generation but also through the glyoxylate shunt and thus provides the cells with metabolic flexibility in the formation of succinate.
Increasing NADH oxidation reduces overflow metabolism in Saccharomyces cerevisiae
Results indicate that NADH oxidase localizes in the cytosol, whereas alternative oxidase is directed to the mitochondria, which indicates that the Crabtree effect is due to limited respiratory capacity or is caused by glucose-mediated repression of respiration.
Overflow Metabolism in Escherichia coli during Steady-State Growth: Transcriptional Regulation and Effect of the Redox Ratio
The role of the NADH/NAD ratio (redox ratio) in overflow metabolism is reported on and a strong correlation between acetate formation and this redox ratio is demonstrated using steady-state chemostat cultures.
Homolactate Fermentation by Metabolically Engineered Escherichia coli Strains
ABSTRACT We report the homofermentative production of lactate in Escherichia coli strains containing mutations in the aceEF, pfl, poxB, and pps genes, which encode the pyruvate dehydrogenase complex,
A co-fermentation strategy to consume sugar mixtures effectively
It is demonstrated that the process is able to adapt to changing concentrations of these two sugars, and therefore holds promise for the conversion of variable sugar feed streams, such as lignocellulosic hydrolysates.
Metabolic Analysis of Escherichia coliin the Presence and Absence of the Carboxylating Enzymes Phosphoenolpyruvate Carboxylase and Pyruvate Carboxylase
Results show that when PPC or PYC is expressed, the metabolic network adapts by altering the flux to lactate and the molar ratio of ethanol to acetate formation, which indicates that during anaerobic metabolism the pyc+strain had a 34% greater specific glucose consumption rate, a 37% greaterspecific rate of ATP formation, and a 6% greater Specific growth rate compared to the ppc+ strain.
Toxicity of Glucosinolates and Their Enzymatic Decomposition Products to Caenorhabditis elegans.
Results indicated that allyl isothiocyanate is nearly three orders of magnitude more toxic to Caenorhabditis elegans than the corresponding glncosinolate, suggesting isothuocyanates formation would improve nematode control from application of glucosinolates.