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Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
TLDR
The efficacy of this cDNA cloning strategy was demonstrated by isolating cDNA clones of mRNA from int-2, a mouse gene that expresses four different transcripts at low abundance, the longest of which is approximately 2.9 kilobases. Expand
Analysis of mouse Evx genes: Evx-1 displays graded expression in the primitive streak.
TLDR
It is speculated that Evx-1 plays a role in the dorsoventral specification of mesodermal cell fate, based on fate-mapping studies indicating that different types of mesoderm emerge from different regions of the primitive streak and the observation that high levels of expression are localized to the region that will give rise to extraembryonic and ventral mesmoderm. Expand
Comparative anatomy of the human APRT gene and enzyme: nucleotide sequence divergence and conservation of a nonrandom CpG dinucleotide arrangement.
TLDR
Comparison between human and mouse APRT gene nucleotide sequences reveals a high degree of homology within protein coding regions but an absence of significant homology in 5' flanking, 3' untranslated, and intron sequences, suggesting that there may be selection for CpG dinucleotides in these regions and that their maintenance may be important for AP RT gene function. Expand
Functional Analysis of a Phosphatidic Acid Binding Domain in Human Raf-1 Kinase
TLDR
It is demonstrated that a functional phosphatidate binding site is necessary for Raf-1 function in embryonic development using a combination of alanine-scanning and deletion mutagenesis to identify the critical amino acid residues in Raf- 1 necessary for interaction with phosphatidic acid. Expand
Nucleotide sequence and organization of the mouse adenine phosphoribosyltransferase gene: presence of a coding region common to animal and bacterial phosphoribosyltransferases that has a variable
We have determined the nucleotide sequence of a functional mouse adenine phosphoribosyltransferase (APRT) gene and its cDNA. The amino acid sequence of the enzyme is deduced from an open readingExpand
Cloning of a functional human adenine phosphoribosyltransferase (APRT) gene: Identification of a restriction fragment length polymorphism and preliminary analysis of DNAs from APRT-deficient families
TLDR
A complete human APRT gene has been isolated from a lambda phage genomic library using cloned mouse APRT DNA as a probe and no gross deletions or rearrangements were revealed, nor the Taq1 polymorphism. Expand
Identification of DNA sequences required for mouse APRT gene expression.
TLDR
No upstream anti-sense transcripts were detected in either mouse CAK or liver cells, confirming that the mouse aprt promoter, unlike some other GC-rich promoters appears not to support bidirectional transcription. Expand
An unusual adenine phosphoribosyltransferase pseudogene is syntenic with its functional gene and is flanked by highly polymorphic DNAs.
TLDR
Double digests of BALB/c and A/J genomic DNAs revealed complex polymorphisms on both sides of the pseudogene and suggest that this region of mouse chromosome 8 may be a region active for mutation or recombination. Expand
An unusual adenine phosphoribosyltransferase pseudogene is syntenic with its functional gene and is flanked by highly polymorphic DNAs
A mouse adenine phosphoribosyltransferase (aprt) pseudogene that had previously been recovered from a BALB/c sperm DNA library possessed several unusual features. Its nucleotide sequence, like thatExpand
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