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Detecting substeps in the rotary motors of FoF1-ATP synthase by Hidden Markov Models
TLDR
The basic prerequisites for the experimental single-molecule FRET data are defined that allow for discrimination between a 120o stepping mode or a 36o substep rotation mode for the proton-driven Fo motor of ATP synthase.
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Regulatory conformational changes of the epsilon subunit in single FRET-labeled FoF1-ATP synthase
TLDR
Labeling the C-terminal domain of epsilon and the gamma subunit specifically with two different fluorophores for single-molecule Foerster resonance energy transfer (smFRET) allowed monitoring of the conformation of ePSilon in the reconstituted enzyme in real time.
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Multistage magnetic and electrophoretic extraction of cells, particles and macromolecules.
TLDR
The theoretical underpinnings of these separations are crucial to their success and to the identification of their advantages over other separation processes in particular applications, so mathematical modeling is stressed here, presenting its own models while also reviewing models reported in the literature.
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Monitoring transient elastic energy storage within the rotary motors of single FoF1-ATP synthase by DCO-ALEX FRET
TLDR
The detection of reversible elastic deformations between the rotor parts of Fo and F1 is reported and the maximum angular displacement during the load-free rotation is estimated using Monte Carlo simulations.
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Manipulating freely diffusing single 20-nm particles in an Anti-Brownian Electrokinetic Trap (ABELtrap)
TLDR
An ABELtrap based on a laser focus pattern generated by a pair of acousto-optical beam deflectors and controlled by a programmable FPGA chip is presented, which increased observation times of a single particle by a factor of 1000.
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Crystal structure of the non-catalytic B subunit of A-type ATPase from M. mazei Go1
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