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Guidelines for the use and interpretation of assays for monitoring autophagy
TLDR
These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. Expand
Inhibition of Trypanosoma brucei Gene Expression by RNA Interference Using an Integratable Vector with Opposing T7 Promoters*
TLDR
A vector for in vivo tetracycline-inducible synthesis of double-stranded RNA (dsRNA) in stably transformed cells is described and one striking phenotype was the loss of kinetoplast DNA after interference with expression of a topoisomerase II. Expand
Glycolysis modulates trypanosome glycoprotein expression as revealed by an RNAi library
TLDR
The data suggest that T.brucei ‘senses’ changes in glucose level and modulates procyclin expression accordingly, and is supported by observation of a similar upregulation of GPEET‐procyclin when parental cells were grown in medium depleted of glucose. Expand
Cloning of a Novel Inosine-Guanosine Transporter Gene fromLeishmania donovani by Functional Rescue of a Transport-deficient Mutant*
TLDR
Results authenticate genetically and biochemically that LdNT2 is a novel nucleoside transporter with an unusual and strict specificity for inosine and guanosine. Expand
The Role of Plasmodium falciparum Food Vacuole Plasmepsins*
TLDR
The results suggest substantial functional redundancy in plasmepsin genes in the food vacuole of Plasmodium falciparum and have important implications for the design of antimalarial drugs. Expand
Intramitochondrial Location and Dynamics of Crithidia fasciculata Kinetoplast Minicircle Replication Intermediates
TLDR
Fluorescence in situ hybridization revealed that before their replication, minicircles are released vectorially from the network face nearest the flagellum, resulting in the kinetoflagellar zone [KFZ], which is thought to take place within the antipodal sites of replication. Expand
Cloning of Leishmania nucleoside transporter genes by rescue of a transport-deficient mutant.
TLDR
Results establish genetically and biochemically that both LdNT1 genes encode functional adenosine/pyrimidine nucleoside transporters. Expand
Plasmodium Food Vacuole Plasmepsins Are Activated by Falcipains*
TLDR
These studies reveal that plasmepsin processing occurs primarily via the falcipains; however, if falCipain activity is blocked, autoprocessing can take place, serving as an alternate activation system, establishing a further level of redundancy between the protease families involved in Plasmodium hemoglobin degradation. Expand
Functional expression of a myo-inositol/H+ symporter from Leishmania donovani
TLDR
It is shown that the biosynthetic precursor for these abundant glycolipids, myo-inositol, is translocated across the parasite plasma membrane by a specific transporter that is structurally related to mammalian facilitative glucose transporters. Expand
A Trypanosome Mitochondrial RNA Polymerase Is Required for Transcription and Replication*
TLDR
The identification and functional characterization of a mitochondrial RNA polymerase (mtRNAP) from Trypanosoma brucei is reported, the firsttrans-acting factor involved in kinetoplast mitochondrial transcription to be identified. Expand
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