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Mutation of the ptsG Gene Results in Increased Production of Succinate in Fermentation of Glucose byEscherichia coli
TLDR
The physiological characteristics of the spontaneous and null mutants were consistent with loss of function of the ptsG gene product; the mutants possessed greatly reduced glucose phosphotransferase activity and lacked normal glucose repression. Expand
Enhanced production of succinic acid by overexpression of phosphoenolpyruvate carboxylase in Escherichia coli
TLDR
Fermentative production of succinic acid from glucose by Escherichia coli was significantly increased by overexpression of phosphoenolpyruvate carboxylase, making succinic Acid the major fermentation product by weight. Expand
Production of succinic acid through overexpression of NAD(+)-dependent malic enzyme in an Escherichia coli mutant
TLDR
The constructed pathway represents an alternative metabolic route for the fermentative production of dicarboxylic acids from renewable feedstocks and was analyzed kinetically in the forward and reverse directions. Expand
Protein production and purification
TLDR
This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators. Expand
A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site.
TLDR
A new LIC vector is developed, pMCSG7, that incorporates the tobacco etch virus (TEV) protease cleavage site into the leader sequence and includes a pair of adjacent, unique restriction sites that will allow insertion of additional modules between the his-tag and the Cleavage site of the leader sequences to generate a family of vectors suitable for high-throughput production of proteins. Expand
A novel fermentation pathway in anEscherichia coli mutant producing succinic acid, acetic acid, and ethanol
TLDR
The data support the emergence in E.coli of a novel succinic acid:acetic acid:ethanol fermentation pathway, and the mutant strain named AFP111, fermented glucose more slowly than did its wild-type ancestor, strain W1485, and generated a very different spectrum of products. Expand
An expression vector tailored for large-scale, high-throughput purification of recombinant proteins.
TLDR
It was found that the dual tag, his(6)-tag-maltose-binding protein (MBP), intended to facilitate purification and enhance proteins' solubility, disrupted such a pipeline, requiring additional screening and purification steps. Expand
The multisubunit active site of fumarase C from Escherichia coli
TLDR
The crystal structure of the tetrameric enzyme, fumarase C from Escherichia coli, has been determined to a resolution of 2.0 Å and has sequence homology to the eukaryotic fumarases, aspartase, arginosuccinating lyase, adenylosuccinate lyase and δ-crystallin. Expand
Transformation of Fatty Acids Catalyzed by Cytochrome P450 Monooxygenase Enzymes of Candida tropicalis
TLDR
To determine the relative activity of each of these enzymes and their contribution to diacid formation, both cytochrome P450s were expressed separately in insect cells in conjunction with the C. tropicalis cyto chrome P450 reductase (NCP) and microsomes prepared from these cells were analyzed for their ability to oxidize fatty acids. Expand
Expression of Ascaris suum malic enzyme in a mutant Escherichia coli allows production of succinic acid from glucose.
TLDR
Under the appropriate culture conditions, expression of malic enzyme allowed the fermentative dissimilation of glucose by Escherichia coli NZN111, a strain that is unable to grow fermentatively because of inactivation of the genes encoding pyruvate dissimilation. Expand
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