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[1] Purification and properties of firefly luciferase
Publisher Summary This chapter discusses purification and properties of firefly luciferase. Firefly luciferase in the presence of luciferin (d-LH2), adenosine triphosphate (ATP)-Mg, and molecularExpand
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Bioluminescence and chemiluminescence
Since the publication of "Bioluminescence and Chemiluminescence, Part B", genes have been cloned that encode luciferases from an array of bioluminescent organisms, novel applications of these genesExpand
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Applications of the Firefly Luciferase as a Reporter Gene
In the last decade, major advances have been made in unraveling the complexities of gene regulation in cells from a variety of organisms. Among the important tools in the arsenal of techniques usedExpand
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[1] Cloning firefly luciferase
Publisher Summary This chapter discusses determination of cloning firefly luciferase. Firefly luciferase catalyzes the ATP-dependent oxidative decarboxylation of luciferin (LH 2 ) resulting in theExpand
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A bioluminescence assay for total 3α-hydroxy bile acids in serum using immobilized enzymes
Abstract A bioluminescence assay for bile acids was developed using a co-immobilized 3α-hydroxysteroid dehydrogenase, diaphorase, and bacterial luciferase. The assay was specific for bile acidsExpand
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Chemical and Enzymatic Mechanisms of Firefly Luminescence
The chemistry of firefly luminescence is gradually being resolved. After a number of years of intensive work by a large number of investigators it is now possible to propose a reasonable organicExpand
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A bioluminescent assay for 12-α-hydroxy bile acids using immobilized enzymes
Abstract A bioluminescent assay for 12-α-hydroxy bile acids was developed using enzymes coimmobilized onto Sepharose 4B. The immobilized enzymes used were a bacterial 12-α-hydroxysteroidExpand
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