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DNA microarray-based identification of genes controlled by autoinducer 2-stimulated quorum sensing in Escherichia coli.
Bacterial cell-to-cell communication facilitates coordinated expression of specific genes in a growth rate-II and cell density-dependent manner, a process known as quorum sensing. While the discoveryExpand
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Cas5d protein processes pre-crRNA and assembles into a cascade-like interference complex in subtype I-C/Dvulg CRISPR-Cas system.
Clustered regularly interspaced short palindromic repeats (CRISPRs), together with an operon of CRISPR-associated (Cas) proteins, form an RNA-based prokaryotic immune system against exogenous geneticExpand
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Folding quality control in the export of proteins by the bacterial twin-arginine translocation pathway
To examine the relationship between folding and export competence by the twin-arginine translocation (Tat) pathway we analyzed the subcellular localization of fusions between a set of eight putativeExpand
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Phage shock protein PspA of Escherichia coli relieves saturation of protein export via the Tat pathway.
Overexpression of either heterologous or homologous proteins that are routed to the periplasm via the twin-arginine translocation (Tat) pathway results in a block of export and concomitantExpand
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Monitoring GFP-operon fusion protein expression during high cell density cultivation of Escherichia coli using an on-line optical sensor.
Synthesis of an operon fusion protein was investigated in batch and fed-batch cultures at high cell densities of recombinant Escherichia coli JM105 [pBAD-GFP::CAT]. Glucose-limited growth wasExpand
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Engineered bacterial outer membrane vesicles with enhanced functionality.
We have engineered bacterial outer membrane vesicles (OMVs) with dramatically enhanced functionality by fusing several heterologous proteins to the vesicle-associated toxin ClyA of Escherichia coli.Expand
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DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency
Synthetic scaffolds that permit spatial and temporal organization of enzymes in living cells are a promising post-translational strategy for controlling the flow of information in both metabolic andExpand
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Double-stranded Endonuclease Activity in Bacillus halodurans Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated Cas2 Protein*
Background: Cas2 is universally conserved and essential for new CRISPR spacer acquisition. Results: Bha_Cas2 uses a single metal ion to cleave dsDNA and is likely activated by a pH-dependentExpand
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Laboratory Evolution of Fast-Folding Green Fluorescent Protein Using Secretory Pathway Quality Control
Green fluorescent protein (GFP) has undergone a long history of optimization to become one of the most popular proteins in all of cell biology. It is thermally and chemically robust and produces aExpand
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Mapping stress-induced changes in autoinducer AI-2 production in chemostat-cultivated Escherichia coli K-12.
Numerous gram-negative bacteria employ a cell-to-cell signaling mechanism, termed quorum sensing, for controlling gene expression in response to population density. Recently, this phenomenon has beenExpand
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