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Rapid Isolation of DNA from Dry and Fresh Samples of Plants Producing Large Amounts of Secondary Metabolites and Essential Oils
- S. Khanuja, A. Shasany, M. Darokar, Sushil Kumar
- BiologyPlant Molecular Biology Reporter
- 1 March 1999
The essential steps of a rapid DNA isolation protocol that can be used for diverse medicinal and aromatic plants, which produce essential oils and secondary metabolites such as alkaloids, flavanoids, phenols, gummy polysaccharides, terpenes and quinones are described.
Essential oil constituents and RAPD markers to establish species relationship in Cymbopogon Spreng. (Poaceae)
Synergistic effect of Glomus mosseae and nitrogen fixing Bacillus subtilis strain Daz26 on artemisinin content in Artemisia annua L.
Membrane-damaging potential of natural L-(−)-usnic acid in Staphylococcus aureus
- V. Gupta, S. Verma, M. Darokar
- Biology, MedicineEuropean Journal of Clinical Microbiology…
- 3 August 2012
The results obtained in this study showed that natural L-(−)-usnic acid exerts its antibacterial activity against MRSA by disruption of the cell membrane, making it a probable candidate for treating S. aureus infections.
A promising anticancer and antimalarial component from the leaves of Bidens pilosa.
Bioactivity-guided fractionation of different extracts of B. pilosa leaf showed potential in vitro anticancer and antimalarial activity and led to the identification of a potential marker compound, phenyl-1,3,5-heptatriyne.
Potential of rosemary oil to be used in drug-resistant infections.
- Suaib Luqman, G. Dwivedi, M. Darokar, A. Kalra, S. Khanuja
- Biology, MedicineAlternative therapies in health and medicine
- 1 September 2007
The findings suggest that characterization and isolation of the active compound(s) from the rosemary oil may be useful in counteracting gram-positive bacterial, fungal, and drug-resistant infections.
Antimicrobial activity of Eucalyptus citriodora essential oil
The findings of the pilot study suggest that characterization and isolation of the active phytoceutical(s) from the E. citriodora oil may provide a valuable antimicrobial agent for counteracting fungal and drug-resistant infections.
Antimycobacterial Activity of Lichens
Ethanol extracts of nine lichen species, namely Everniastrum cirrhatum, were evaluated for antimycobacteral properties against Mycobacterium tuberculosis and the virulent strain of M. tuberculosis did not exhibit activity at the maximum tested concentration of 1000 µg/mL.
Chemical and Biological Diversity in Fourteen Selections of Four Ocimum Species
- B. R. Rao, S. Kothari, D. K. Rajput, Rajendra P. Patel, M. Darokar
- Chemistry, MedicineNatural product communications
- 1 November 2011
The essential oils exhibited broad spectrum antibacterial and antifungal activities and the bacterial species Bacillus subtilis, Staphylococcus aureus, Streptococcus mutans, and Enterococcus faecalis, and the fungal species Epidermophyton floccosum, Microsporum gypseum, and Sporothrix schenckii were more sensitive to the essential oils.