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Epsin: inducing membrane curvature.
To perform all these functions, epsin, apart from interacting with PtdIns(4,5)P2 via its ENTH domain, also engages in several protein interactions with different components of the clathrin-mediated endocytic system. Expand
A Dipeptide Metalloendoprotease Substrate Completely Blocks the Response of Cells in Culture to Cholera Toxin*
  • M. D. De Wolf
  • Medicine
  • The Journal of Biological Chemistry
  • 29 September 2000
Several lines of evidence indicate that a perturbation of intracellular Ca2+ homeostasis by Cbz-Gly-Phe-NH2 is not responsible for the inhibitory effect of the dipeptide, compatible with a role of metalloendoprotease activity in the intrACEllular trafficking and processing of CT. Expand
Structural features of the binding site of cholera toxin inferred from fluorescence measurements.
The dependence on pH of the fluorescence of cholera toxin and its A and B subunits has been studied at 25 degrees C and indicates that the tryptophan fluorescence is only moderately altered by ionizable groups displaying a pKa in the range 4 to 9. Expand
Nucleotide sequence analysis of the CT operon of the Vibrio cholerae classical strain 569B.
The complete nucleotide sequence of the Vibrio cholerae classical strain 569B was determined. The results prove the exactness of the amino acid CT B sequence published by Takao et al. (1985, Eur. J.Expand
Interaction of a cholera toxin derivative containing a reduced number of receptor binding sites with intact cells in culture.
The results are consistent with the view that CT receptor recognition domains are shared between adjacent beta-chains, that pentavalent binding appears not to be essential for cytotoxicity and that in the cell types studied intracellular processing of CT, hCT is involved. Expand
Subcellular structure of bovine thyroid gland. A study on bovine thyroid membranes by buoyant-density-gradient centrifugation in a B-XIV zonal rotor.
A combined mitochondrial and light mitochondrial fraction and a microsomal fraction were isolated from bovine thyroid gland and fractionated further in a B-XIV zonal rotor, resulting in the formation of a single zone at intermediate densities (agg of membranes?). Expand
Phospholipases A1 and A2 in bovine thyroid.
Enzyme assays performed on subcellular fractions suggest the soluble phospholipases to be of lysosomal origin and the solubilized phospholIPase A2 activity of homogenate sediment toBe of microsomal origin. Expand
Thyroid membrane ADP ribosyltransferase activity. Stimulation by thyrotropin and activity in functioning and nonfunctioning rat thyroid cells in culture.
Bovine thyroid membranes possess both ADP ribosyltransferase and NAD glycohydrolase activities with the same Km values for NAD and the same pH optima. In intact membranes, the ADP ribosyltransferaseExpand
Subcellular structure of bovine thyroid gland. The localization of the peroxidase activity in bovine thyroid.
A localization of guaiacol peroxidase in rough-endoplasmic-reticulum membranes (or in membranes related to them) seems very likely. Expand
Subcellular structure of bovine thyroid gland. VII. A study on the distribution of bovine thyroid plasma membranes by density gradient centrifugation in zonal rotors.
It is concluded from approach A that plasma membranes from bovine thyroid tissue are heterogeneous and results in a subdivision of membrane material in four distinct regions. Expand