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Characterization of amino acid pools in the vacuolar compartment of Saccharomyces cerevisiae
Intact vacuoles are released from spheroplasts of Saccharomyces cerevisiae by means of a gentle mechanical disintegration method. They are purified by centrifugation in isotonic density gradientsExpand
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Characterization of a specific transport system for arginine in isolated yeast vacuoles.
The transport of L-arginine was studied in isolated vacuoles of Saccharomyces cerevisiae. A centrifugation method allowed rapid separation of the fragile vacuoles from the incubation media so thatExpand
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Asymmetric distribution of Concanavalin A binding sites on yeast plasmalemma and vacuolar membrane
Isolated vacuoles of Saccharomyces cerevisiae did not bind Concanavalin A (labelled with tritium or with a fluorescent dye) unless the vacuoles were rendered permeable and their inner membraneExpand
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Localization of polyphosphate in vacuoles of Saccharomyces cerevisiae
Virtually all of the polyphosphate (PP) present in yeast protoplasts can be recovered in a crude particulate fraction if polybase-induced lysis is used for disrupting the protoplasts. This fractionExpand
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Involvement of Lipid-linked Oligosaccharides in Synthesis of Storage Glycoproteins in Soybean Seeds.
Membrane preparations from developing soybean (var. Prize) cotyledon tissue, at the time of synthesis of storage glycoproteins, catalyze the sequential assembly of lipid-linked oligosaccharides fromExpand
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Subcellular distribution of membrane-bound glycosyltransferases from pea stems.
Particulate and subcellular membrane preparations from growing regions of etiolated pea stems catalyse the transfer of sugars from UDP-[14C]glucose and GDP-[14C]mannose to a variety of endogenousExpand
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Polybase induced lysis of yeast spheroplasts
The polybasic macromolecules DEAE-dextran (diethylaminoethyl-dextran, molecular weight 500 000) and poly-dl-lysine (molecular weight 30 000–70 000) were adsorbed with a high affinity by spheroplastsExpand
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The assembly of lipid-linked oligosaccharides in plant and animal membranes.
Membrane preparations from growing regions of pea stems and actively-dividing mouse L-cells form lipid-linked saccharides from GDP-mannose and UDP-N-acetylglucosamine. These lipids have propertiesExpand
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Action of proteinases on the arginine transport system of purified vacuoles from Saccharomyces cerevisiae.
Abstract Thermolysin, a commercial bacterial proteinase, greatly activated the arginine transport system of isolated yeast vacuoles. Pronase had the same effect at low concentrations, but rapidlyExpand
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Sequestration of arginine by polyphosphate in vacuoles of yeast (Saccharomyces cerevisiae)
Isolated and purified vacuoles from yeast protoplasts contain the bulk of the cellular pool of arginine. The arginine is firmly retained in the isolated vacuoles despite of the presence of a permeaseExpand
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