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RAPD Analysis Provides Insight into the Biology and Epidemiology of Uncinula necator.
TLDR
A polymerase chain reaction primer pair, derived from a RAPD fragment specific to the Indian isolates, proved to be suitable for field studies and may represent a genetically isolated biotype of U. necator.
Isolation and characterization of a Vitis vinifera transcription factor, VvWRKY1, and its effect on responses to fungal pathogens in transgenic tobacco plants.
TLDR
Biochemical analysis indicates that Vv WRKY1 specifically interacts with the W-box in various nucleotidic contexts, consistent with a possible role for VvWRKY1 in grapevine defence against fungal pathogens.
A mutation in the 14 alpha-demethylase gene of Uncinula necator that correlates with resistance to a sterol biosynthesis inhibitor
TLDR
The molecular basis of resistance of the obligate biotrophic grape powdery mildew fungus Uncinula necator to sterol demethylation-inhibiting fungicides (DMIs) is investigated and a single mutation, leading to the substitution of a phenylalanine residue for a tyrosine residue at position 136, is found.
Over-Expression of VvWRKY1 in Grapevines Induces Expression of Jasmonic Acid Pathway-Related Genes and Confers Higher Tolerance to the Downy Mildew
TLDR
Results show that VvWRKY1 can increase resistance of grapevine against the downy mildew through transcriptional reprogramming leading to activation of the jasmonic acid signalling pathway.
Benzothiadiazole-primed defence responses and enhanced differential expression of defence genes in Vitis vinifera infected with biotrophic pathogens Erysiphe necator and Plasmopara viticola
TLDR
The temporal evolution of defence-related genes in pre-treated infected leaves suggests that grapevine responses vary depending on the inter- or intra-species variability of pathogens.
Nested Allele-Specific PCR Primers Distinguish Genetic Groups of Uncinula necator
TLDR
In a preliminary study performed with samples from French vineyards in which isolates belonging to genetic groups I and III were present, it is found that a shift from a population composed primarily of group I isolates to a population composition composed of group III isolates occurred during the grapevine growing season.
Microsatellite DNA markers for Plasmopara viticola, the causal agent of downy mildew of grapes
TLDR
Cross-amplification tests on three closely related taxa indicated that two loci could be used in other Oomycetes species, and these microsatellite loci were proved to be useful for population genetic analysis.
At Least Two Origins of Fungicide Resistance in Grapevine Downy Mildew Populations
TLDR
A high population structure was found when the frequency of QoI fungicide resistance haplotypes was assessed in 17 French vineyards, indicating that pathogen populations might be under strong directional selection for local adaptation to fungicide pressure.
Overexpression of VvWRKY2 in tobacco enhances broad resistance to necrotrophic fungal pathogens.
TLDR
Constitutive expression of VvWRKY2 in tobacco reduced the susceptibility of transgenic tobacco to three types of fungal pathogens infecting different parts of the plant: Botrytis cinerea (leaves), Pythium spp.
Ethephon elicits protection against Erysiphe necator in grapevine.
TLDR
Ethephon treatment triggered the protection of grapevine detached leaves and grapevine foliar cuttings against Erysiphe necator, the causal agent of powdery mildew, and these studies emphasize the major role of ethylene in grapevine defense.
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