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Complement factor H as a marker for detection of bladder cancer.
It is demonstrated that FH is the ligand recognized by the BTA TRAK test, a special mechanism for a diagnostic immunoassay based on the combined effect of simultaneous positive and negative signals in a single sample.
Complement factor H or a related protein is a marker for transitional cell cancer of the bladder.
- R. Kinders, T. Jones, G. Hass
- BiologyClinical cancer research : an official journal of…
- 1 October 1998
It is proposed that the expression of proteins with FH-like activities may confer a selective growth advantage to cancer cells in vivo by decreasing complement activity, thus aiding their escape from lysis by immune surveillance.
LNCaP produces both putative zymogen and inactive, free form of prostate‐specific antigen
Our objective was to investigate the presence of prostate specific antigen (PSA) and α‐1‐antichymotrypsin (ACT) mRNA and protein in prostate cancer cell lines, and the complexing characteristics of…
Mechanistic Studies of the Effects of Anti-factor H Antibodies on Complement-mediated Lysis*
It is indicated that it is possible to deconvolute the biochemical roles of factor H in complement by means of appropriate inhibitors, a finding with potentially valuable implications for both basic research and cancer therapy.
A very sensitive coupled luminescent assay for cytotoxicity and complement-mediated lysis.
Prostate-specific antigen: characterization of epitopes by synthetic peptide mapping and inhibition studies.
Examination of the association targets of one anti-PSA polyclonal antibody and 10 anti- PSA monoclonal antibodies established that three of them bind to linear epitopes, but inhibition of PSA enzymatic activity and hindrance of Psa-ACT association by mAbs cannot be used to predict whether the mAbs bind to free PSA, the PSA-ACT complex, or both.
Site-directed mutagenesis of the catalytic residues Asp-52 and Glu-35 of chicken egg white lysozyme.
- B. Malcolm, S. Rosenberg, M. Corey, J. S. Allen, A. de Baetselier, J. Kirsch
- Biology, ChemistryProceedings of the National Academy of Sciences…
The roles of the catalytic active-site residues aspartic acid-52 and glutamic acid-35 of chicken lysozyme have been investigated by separate in vitro mutagenesis of each residue to its corresponding amide to indicate the alterations in catalytic properties may be due in part to binding effects as well as to direct catalytic participation of these residues.
Detection of disseminated prostate cells by reverse transcription‐polymerase chain reaction (RT‐PCR): Technical and clinical aspects
There is considerable interest in the use of RT-PCR for detection and possible quantification of prostate cells in peripheral blood, bone marrow, and lymph node samples from CaP patients, but despite the promise of this method, it is not yet clear whether RT- PCR will prove to be of clinical value in prostatecancer staging.
High-affinity peptide ligands to prostate-specific antigen identified by polysome selection.
- G. Gersuk, M. Corey, E. Corey, J. Stray, G. Kawasaki, R. Vessella
- Biology, ChemistryBiochemical and biophysical research…
- 17 March 1997
The polysome-selection method can be used to produce high-affinity peptide ligands of potential use in diagnostic and therapeutic procedures, and in one case, virtually eliminated non-specific binding.
Characterization of 10 new monoclonal antibodies against prostate‐specific antigen by analysis of affinity, specificity and function in sandwich assays
Physical analysis of the new antibodies has allowed the MAbs to be assigned to binding classes (based on their sandwiching capabilities) and to determine accurate apparent dissociation constants, as well as assigning and characterized 10 anti‐PSA monoclonal antibodies.