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Redox control in trypanosomatids, parasitic protozoa with trypanothione-based thiol metabolism.
Identification of novel bacterial plasminogen‐binding proteins in the human pathogen Mycobacterium tuberculosis
Proteomic analysis together with ligand blotting assays identified several major Plg‐binding spots in Mycobacterium tuberculosis soluble extracts and culture filtrate proteins suggesting that M. tuberculosis posses several Plg receptors suggesting that bound Plg to bacteria surface, can be activated to Plm, endowing bacteria with the ability to break down ECM and basal membranes proteins contributing to tissue injury in tuberculosis.
Validation of Trypanosoma brucei trypanothione synthetase as drug target.
Depletion of the thioredoxin homologue tryparedoxin impairs antioxidative defence in African trypanosomes.
The results prove the essential role of the cTXN and its pivotal function in the parasite defence against oxidative stress and the sensitivity against exogenously generated H2O2 was significantly enhanced.
Trypanothione Synthesis in Crithidia Revisited*
- M. Comini, U. Menge, J. Wissing, L. Flohé
- Biology, ChemistryJournal of Biological Chemistry
- 25 February 2005
Steady-state kinetic analysis of Cf-TryS yielded a pattern that was compatible with a concerted substitution mechanism, wherein the enzyme forms a ternary complex with Mg2+-ATP and GSH to phosphorylate GSH and then ligates the glutathioneyl residue to glutathionylspermidine.
Catalytic mechanism of the glutathione peroxidase-type tryparedoxin peroxidase of Trypanosoma brucei.
- Tanja Schlecker, M. Comini, J. Melchers, T. Ruppert, R. Krauth-Siegel
- Biology, ChemistryThe Biochemical journal
- 1 August 2007
Site-directed mutagenesis revealed that Cys47 and Gln82 are essential for catalysis and replacement of the highly conserved Cys76 by a serine residue resulted in a fully active enzyme species and its role remains unknown.
Determination of acidity and nucleophilicity in thiols by reaction with monobromobimane and fluorescence detection.
Evaluation of the diagnostic value of measuring IgG, IgM and IgA antibodies to the recombinant 16-kilodalton antigen of mycobacterium tuberculosis in childhood tuberculosis.
- M. Imaz, M. Comini, M. Singh
- Medicine, BiologyThe international journal of tuberculosis and…
- 1 November 2001
The detection of anti 16-kDa IgG and IgA may be useful as a complementary technique for the diagnosis of childhood TB and combining responses against other antigens may be a good strategy to improve the performance of this assay.
Insights into the redox biology of Trypanosoma cruzi: Trypanothione metabolism and oxidant detoxification.
The trypanothione system.
Despite overlapping substrate specificities and subcellular localizations, the two types of peroxidases can obviously not substitute for each other which suggests distinct cell-physiological roles.